RedfluorescentLysoView™540andfar-redfluorescentLysoView™633stainlysosomesinlivecells.Far-redLysoView™650iscompatIBLewithsuper-resolutionimagingbySTEDandSIM.

LysoView™dyeslabellysosomesinlivecells,andareavailablein3dyecolors:visibleredLysoView™540,far-redLysoView™633,andfar-redLysoView™650.

LysoView™540isaredfluorogenicdyewithpH-dependentfluorescenceforstainingacidicorganellesinlivecells.LysoView™540belongstoaclassoflysosomotropicdyesthatpossessweaklybasicaminegroups.ItishypothesizedthatprotonationofweakbasesunderacidicconditionscausesthedyestobetrappedinthelowpHcompartmentofthelysosome.Inaddition,thefluorescenceofLysoView™540ispH-sensitive,resultinginhighlyspecificlysosomalstainingwithouttheneedforawashstep.Thedyehasexcitation/emissionat541/634nmforimagingintheCy®3channel.

LysoView™633isafar-redfluorescentdyethatishighlyselectiveforlabelingandtrackingacidicorganellesinlivecells. ComparedtoLysoTracker®DeepRed,LysoView™633offershighlyspecificlysosomalstainingwithnowashsteprequired.Withabsorptionandemissionat634/659nm,thedyecanbedetectedusingaCy®5filterset.

LysoView™650isafar-redfluorescentlysosomestainwith absorption/emissionat650/675nm,whichiscompatiblewithsuper-resolutionimagingbySIMandSTED.

LysoView™540andLysoView™650aresuppliedas1000XstocksolutionsinDMSO.LysoView™633issuppliedasa lyophilizedsolid;afterreconstitutioninwater,eachvialyields100uLof1000Xdyestocksolution.

AlsoseeouruniqueUV-activatable Light-OnLysoView™555,whichisnon-fluorescentuntilitisactivatedbyUVlight.

CyDyeisaregisteredtrademarkofGEHealthcare.

Biotium 5(6)-羧基-X-罗丹明-N-羟基琥珀酰亚胺酯 Biotium 异硫氰酸荧光素酯 Biotium 10-壬基吖啶橙溴化物 Biotium 4-甲基伞形酮磷酸二钠盐三水 Biotium 河豚毒素 Biotium 2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐 Biotium N,N-二甲基甲酰胺 Biotium N-生物素酰基-L-赖氨酸酰肼 Biotium 5-溴-6-氯-3-吲哚基-beta-D-半乳糖苷 Biotium N,N,N',N'-四(2-吡啶甲基)乙二胺 Biotium 腔肠素-H Biotium Α-金环蛇毒 Biotium 5-溴-4-氯-3-吲哚半乳糖苷 Biotium BIOTIN-(AC5)2-OSU Biotium 4',6-二脒基-2-苯基吲哚二盐酸盐 Biotium 4-甲基伞形酮磷酸酯 Biotium 90% (TLC) Biotium 96314-98-6 SULFORHODAMINE G 5873-16-5 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 127770-45-0 HYDROETHIDINE 38483-26-0 SULFORHODAMINE 101 60311-02-6 L-N6-(1-IMINOETHYL)LYSINE DIHYDROCHLORIDE 150403-89-7