BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content even in the presence of detergents.

This kit is based on the reduction of Cu²⁺ to Cu¹⁺ by protein in alkaline solution; monovalent copper ions produced are detected in a concentration-dependent manner. Bicinchoninic acid (BCA) chelates with the reduced copper Cu¹⁺ and forms a water-soluble purple reaction complex that exhibits a strong absorbance at 562 nm. Absorbance is linear over a wide range of protein concentrations between 25-2000 µg/mL.        In general, protein concentrations are estimated with reference to a commonly used protein standard; the kit also includes Bovine Serum Albumin (BSA) at 1.5 mg/mL as a protein standard for a convenient preparation of protein concentration standard curves. 

Kit contains:

• BCA Reagent A (500mL) / Copper Solution B (15mL) / BSA 5x1mL - 1500µg/mL

Storage: Store at 4°C

Shipping condition: The kit is shipped at RT.

 

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自2003年以来，OZ Biosciences为全球科学界和制药行业创建，开发和生产专门用于转染和转导工具的创新分子递送系统。OZ Biosciences专注于体外和体内应用的生物分子（例如DNA，RNA和蛋白质）的输送技术。在过去的几年中，生命科学经历了技术革命。在基因组学，功能基因组学和蛋白质组学领域所取得的进展为更好地理解基本生物学过程和开发下一代药物创造了绝佳的机会。