Overview

The Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. The lysis and binding buffers are specifically designed to minimize co-purification of polysaccharides and polyphenols. Up 96 samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than one hour. The system combines CTAB-based lysis, which eliminates the need for organic solvents, with the convenience of Mag-Bind® Particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, next-generation sequencing, and hybridization applications. There are no organic extractions thereby reducing consumables and decreasing hands-on time to allow multiple samples to be processed in parallel.

Protocol and Resources

Product Documentation & Literature

Product Data

Mag-Bind® Plant DNA DS 96 Kit performs better for most plant types than leading competitor.

Figure 1. Approximately 50 mg leaf sample extracted per sample according to manufacturer’s recommended protocols. DNA concentration determined via fluorescence-based nucleic acid quantification. DNA quantification confirmed via SYBR® qPCR (data not shown). Amount of DNA per mg of leaf sample shown above.

DNA purified using Mag-Bind Plant DNA DS 96 Kit was around 30 kb

Figure 2  The sizes of genomic DNA purified using Mag-Bind kits were determined by electrophoresis. Sample 1 and 2 were gDNA from barley purified with kit M1130 (Mag-Bind Plant DNA DS Kit). 50 kb ladder is the genomic ladder for Agilent TapeStation 2200. The size of gDNA from M1130 is around 30 kb.

DNA Yield Yield From Canola Leaf.

Figure 3.1  Genomic DNA was purified from 50 mg canola leaf with the Mag-Bind Plant DNA DS 96 Kit. DNA concentration determined by optical density measurements with NanoDrop® 2000c. Total elution volume was 100 µL.

High Molecular Weight Genomic DNA purified from Canola Leaf

Figure 3.2  Genomic DNA was purified from 50 mg canola leaf with the Mag-Bind Plant DNA DS 96 Kit. 5 µL eluate DNA was analyzed on a 1% Agarose gel.

PCR Inhibitor-Free DNA – Canola Leaf

Figure 3.3  Genomic DNA was extracted from 50 mg canola leaf using the Mag-Bind Plant DNA DS 96 Kit. 2 µL of Eluted DNA was diluted 10- and 100-fold and used as a template in a 20 µL SYBR® qPCR reaction. The Ct values increased by only 3 cycles per 10-fold dilution, which demonstrates that the template DNA is free of inhibitors.

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