The Perl’s stain is the most commonly used method to detect the presence of iron in biopsy specimens. It depends on the Prussian blue reaction. The original stain formula, known historically (1867) as "Perls" Prussian blue" after its inventor, German pathologist Max Perls (1843-1881), used separate solutions of potassium ferrocyanide and acid to stain tissue, the tissue is first treated with dilute hydrochloric acid to release ferric ions from binding proteins. The freed ions then react with potassium ferrocyanide to form insoluble Prussian blue dye (a complex hydrated ferric ferrocyanide substance) in situ. Hito Perl’s Iron OptimStain™ Kit designed based on the Perl’s stain, this kit makes dramatic improvement of the Perls" Prussian blue technique. The procedures are simplified and the processing time is reduced. This kit delivers stable and improved staining quality. It is a simple solution for your research.

Kit Contents	(for 150 slides)
Solution-1A	125 ml
Solution-1B	125 ml
Solution-2	125 ml
Solution-3 (counterstain)	30 ml
Dropper Bottle (30 ml Solution-3)	1
Staining Jars	2
Iron Positive Control Slides (HTTS0002)	2
User Manual and MSDS	1
Before using Hito Perl’s Iron OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Microtome and light microscopeParaffin embedding equipmentEthanol, Xylene, 4% PFA (Hito Cat# HTSHS0102), double distilled or deionized waterSlides and coverslipsStaining jars for slides washResinous mounting medium Hito Perl’s Iron OptimStain™ Kit Manual and MSDS