Von Kossa staining has been used to demonstrate deposits of calcium in tissues since the 19th century. The method was originally developed by von Kossa, and underwent several modifications. The Von Kossa stain is not specific for calcium itself, instead it stains calcium salts like calcium phosphate and calcium carbonate, The silver ions in the staining solution binds with phosphate or carbonate by replacing calcium in a precipitation reaction. The silver salts are then reduced to black metallic silver under strong light illumination. So Von Kossa stain allows indirect visualization of the calcium deposits in tissue samples.Hito Von Kossa OptimStain™ Kit produces brown to black calcium salts against red cytoplasm and nuclei in just 90 minutes. The developer solution is included in the kit, therefore exposure of the slides to strong light, sunlight, or ultra violet light is not required. This stain kit delivers stable and improved staining quality.For verification of the staining results, Calcium Positive Control Slides are included in the kit (16-18 days mouse embryo).Hito Von Kossa OptimStain™ Kit has been tested extensively on the different tissue sections from several species of animals and it is a simple solution for your research.

Kit Contents	Standard Kit	Small Kit
Solution-1	100 ml	50 ml
Solution-2	250 ml	125 ml
Solution-3	250 ml	125 ml
Solution-4	250 ml	125 ml
Solution-5	30 ml	15 ml
Dropping Bottle for Solution-5	1	1
Staining Jar (12 ml)	4	4
Calcium Control Slide (Positive)	3	1
User Manual and MSDS	1	1
Before using Hito Von Kossa OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat or microtomeDry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized waterStaining jars for slides washResinous mounting mediumLight microscope Hito Von Kossa OptimStain™ Kit Manual and MSDS