Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.

Abbkine Mitochondrion Staining Kits are a set of fluorescence imaging tools to label mitochondria of live cells. This kit uses a proprietary mitochondrial orange fluorescent probe (Ex/Em=579/599 nm), which, like other probes, the kit uses a proprietary dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient. MitoOrange, a hydrophobic compound, easily permeates intact living cells and can be retained after formaldehyde fixation and permeabilization. The dye is suitable for double labeling experiments, and its orange fluorescent probes is well distinguished from other green fluorescent probes. The fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. This kit is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.