| Artikelnummer | 515-035-003 |
|---|---|
| Wirtsspezies | Schaf |
| IgG-Form | IgG Gesamtmolekül |
| Spezies-Reaktivität | Maus |
| Spezifität | IgG (H+L) |
| Isotyp | Ig |
| Klonalität (Mono-/Polyklonal) | polyklonal |
| Anwendung | ELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot |
| Konjugation | Horseradish Peroxidase (HRP) |
| Keine Kreuzreaktivität (MinX) mit | keine |
| Verdünnung | ELISA 1:5.000 – 1:100.000, Histo-/Zytochemie 1:500 – 1:5.000, Western Blot (ECL): 1:10.000 – 1:200.000, Western Blot (non-ECL): 1:5.000 – 1:100.000 |
| Format | 15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), Lyophilisat |
| Zweckbestimmung | nur für Forschungszwecke |
| Produktlinie / Thema | Jackson ImmunoResearch |
| Hersteller / Marke | Jackson ImmunoResearch |
- 515-035-003 – Datenblatt
Conjugate
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
