This high-throughput method measures residual E. coli host cell DNA. It uses our proprietary extraction procedure, which isolates residual DNA without the use of iodine or glycogen. Cell line-specific E. coli DNA calibrators are included, and upon completion of extraction, samples are reacted with PicoGreen^® solution. Time to completion is under 2 hours for multiple samples and multiple deep well plates.Each kit supplies enough reagents for the analysis of 96 samples.