CLIA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM)

SCA002Hu

96T

Sandwich CLIA Kit

CLIA

Human

CLIA Kits

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Activated Leukocyte Cell Adhesion Molecule (ALCAM) level in the sample or standard.This assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM). No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.

Detection Range: 27.43-20000pg/mL The standard curve concentrations used for the CLIA?s were 20000pg/mL, 6666.67pg/mL, 2222.22pg/mL, 740.74pg/mL, 246.91pg/mL, 82.3pg/mL, 27.43pg/mL

Sensitivity: The minimum detectable dose of this kit is typically less than12.9pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 4.5hours

Cloud-Clone Corp.

CLIA Kits