Chromogenicinsituhybridization(ISH)permitsthevisualidentificationofspecificmRNAorDNAnucleicacidsequencesintissues.Followingapplicationoftheprobe,thepresenceofatargetnucleicacidisvisualizedbythesequentialapplicationofasecondaryreagentthatbindsthedigoxigeninlabeledprobe,followedbyatertiaryenzyme-antibodyconjugate,andachromogenreagent,toproduceacoloredreactionproductthatisvisIBLebylightmicroscopy.
ISHHRPDetectioniscomprisedofamouseanti-digoxigeninantibodyasasecondaryreagentandanHRPenzyme-antibodyconjugateasatertiaryreagent,suitableforthedetectionofdigoxigeninlabeledprobesaspartofanISHstainingprocedureontheONCOREAutomatedSlideStainer.ISHHRPDetectionisprovidedready-to-useandisintendedtobeappliedasdefinedbythestainingprotocolsontheONCOREAutomatedSlideStainer.
| Format | ONCORE |
|---|---|
| IntendedUse | IVD |
| Volume | 60Tests |
References:
1.BeckRC,etal.Automatedcolorimetricinsituhybridization(CISH)detectionofimmunoglobulin(Ig)lightchainmRNAexpressioninplasmacell(PC)dyscrasiasandnon-Hodgkinlymphoma.DiagnMolPathol.2003Mar;12(1):14-20.
2.ShibataY,etal.AssessmentofdecalcifyingprotocolsforthedetectionofspecificRNAbynon-rADIoactiveinsituhybridizationincalcifiedtissues.HistochemCellBiol.2000Mar;113(3):153-9.
3.WilkinsonDG.InSituHybridization:APracticalApproach(PracticalApproachSeries).2ndEd.Oxford:OxfordUniversityPress,1999.
4.NuovoGJ.InSituMolecularPathologyandCo-ExpressionAnalyses.1stEd.SanDiego:AcademicPress,2013.
5.ClinicalandLaboratoryStandardsInstitute(CLSI).ProtectionofLaboratoryWorkersfromOccupationallyAcquiredInfections;ApprovedGuideline-FourthEditionCLSIdocumentM29-A4Wayne,PA2014.
