Biocare’sPromARKTMseriesintroducesMouse-on-FarmaHRP-Polymerdetection.TheMouse-on-FarmaHRP-Polymerisformulatedtodetectmousemonoclonalantibodiesandisaffinitypurifiedforminimalcross-reactivitytocow,horse,pigandsheepIgGproteins.Theadvantagesofthispolymertechnologyareincreasedsensitivity,reductionofIHCsteps(noavidin/biotinblockwithlink/enzymeconjugates),minimalcross-reactivitytoendogenousIgGthatallowsmultiplespeciesflexibility,andinmostcases,tissuesdonotrequireaproteinblock.TheMouse-on-Farmadetectioncanbeusedonparaffin-embeddedtissuesandcanbeperformedmanuallyoronautomatedstainingplatforms.ThisdetectionmaybeusedwithanyofBiocareMedical’sretrievalsolutions(Reveal/Borg/Diva)ordigestiveenzymes.TemperaturedependentprotocolscanbeconvenientlyperformedusingBiocare’sDecloakingChamber.TheMouse-on-FarmaHRP-PolymermaybecombinedwiththeRabbit-on-FarmaHRP-PolymertoprepareauniversalHRP-PolymerdetectionthatwilllabelbothmouseandrabbitantibodieswithHRP.RefertoTechnicalNote#4foradditionalinformation.TheMouse-on-FarmaHRP-PolymermaybecombinedwiththeRabbit-on-FarmaAP-PolymertoprepareadoublestainpolymerdetectionthatwilllabelthemouseantibodywithHRPandtherabbitantibodywithAP.RefertoTechnicalNote#5foradditionalinformation.
