Product Description: Recombinant HEK293 cell line expressing human IKCA1, also known as KCNN4 (Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4), Genbank Accession No. NM_002250. Format: Each vial contains 2 X 10^6 cells in 1 ml of 10% DMSO.
Mycoplasma Testing: The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species. Introduction: IKCA1 is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes.
References: Ghanshani S et al. (2000) Up-regulation of the IKCa1 potassium channel during T-cell activation. J Biol Chem. 275(47): 37137-37149 Hoffman JF et al. (2003) The hSK4 (KCNN4) isoform is the Ca2+-activated K+ channel (Gardos channel) in human red blood cells. PNAS 100 (12): 7366-7371
de-Allie FA et al. (1996) Characterization of Ca(2+)-activated 86Rb+ fluxes in rat C6 glioma cells: a system for identifying novel IKCa-channel toxins. Br J Pharmacol. 117(3):479-487
Terstappen GC et al. (2003) The antidepressant fluoxetine blocks the human small conductance calcium-activated potassium channels SK1, SK2 and SK3. Neurosci Lett. 346(1-2):85-88
