A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder, Solubility without autoclaving: Light tan, incomplete, Solubility with autoclaving: Light tan, clear, complete, pH with autoclaving: As Reported, Protocol: Cooling the N1000 solution after autoclaving: , Place the sterile N1000 solution into a 58°C water bath. N1000 solution needs to completely cool to the desired temperature (58°C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58°C, place flask(s) onto stir plate(s). Maintain temperature at 58°C. Add the following:, a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution, b)Sterile 1M CaCl2: 1ml/liter N1000 solution, c) Sterile 1M MgSO4: 1ml/liter N1000 solution, Allow the solution to mix for five minutes before starting to pour plates to ensure that a homogeneous mixture has been achieved., *1M Phosphate buffer is prepared by dissolving 108.3g KH2PO4 and 35.6g K2HPO4 in ddH2O. QS to 1L).