Transfectamine(TM) 5000 Transfection Reagent is a powerful and versatile transfection reagent for the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. It can effectively transfect a variety of payloads into a variety of adherent and suspension cell lines. It can be used for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies. It offers consistently high transfection efficiency in a wide variety of adherent and suspension cell lines, including difficult-to-transfect cells. The low toxicity of Transfectamine(TM) 5000 also allowed higher viability of transfected cells. Transfectamine(TM) 5000 is easier to use compare to most other transfection reagents and does not require special medium.