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品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description
A robust, efficient, proofreading enzyme that gives increased fidelity in high-yield PCR, for use in all routine cloning applications.
Product Highlights
- Efficient – highly productive target amplification and removal of 3’ A overhangs
- Accurate – possesses 3’ - 5’ proofreading exonuclease activity that delivers an error rate of 3.0 x 10-6 for increased PCR fidelity versus Taq DNA polymerase
- Sensitive – high-yield amplification from limiting amounts of human, animal and plant template DNA
- Robust – developed for reliable amplification of even the most challenging targets, including genomic DNA and GC-rich targets
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samples
- Convenient – advanced buffering system minimizes the requirements for PCR optimization, thereby reducing time-to-results and eliminating the cost of unnecessary repeats
Product Description
ACCUZYME™ is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME has an error-rate of 3.0 x 10-6 and results in blunt-ended amplicons up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis.
ACCUZYME is supplied with a buffering system that provides ideal conditions for most PCR assays. Consequently, the cost and effort typically associated with optimizing assay performance is often eliminated. In circumstances where further optimization is required to improve PCR specificity and/or yield, ACCUZYME includes an additional vial of MgCl2.
Applications
- High-fidelity PCR
- Routine cloning applications requiring increased PCR yield
- Blunt-end cloning
- Site-directed mutagenesis
