The Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).

Test material includes:

• Cell culture derived material
• Soluble sGAGs from fluids, such as synovial fluid, urine.
• Cartilage
• Connective tissue / tumours

Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.

The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.

The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.

Please note:

The assay is not suitable for small sulfated disaccharide fragments or for the samples containing alginates, as these contain uronic acid.

Biocolor的糖胺聚糖测定Blyscan™所述Blyscan™糖胺测定法是用于硫酸化蛋白聚糖和糖胺聚糖，聚糖（sGAG）的分析的定量染料结合法。测试材料包括：细胞培养衍生材料来自滑液，尿液等液体的可溶性sGAG。软骨结缔组织/肿瘤可溶性sGAG可以直接测定，固体物质需要初步的木瓜蛋白酶提取。该测定法可用于测量总sGAG含量，也可用于确定测试样品中的O-和N-硫酸化的糖胺聚糖比率。测定中使用的染料标记是1,9-二甲基亚甲基蓝，并且该染料在为蛋白多糖的硫酸化多糖组分或无蛋白的硫酸化糖胺聚糖链提供特定标记的条件下使用。