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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Product Description:Product Features - Prestained Protein Ladder
Broad range (10-250 kD)Dual-Color or Tri-ColorHigh size precision and lot consistencyClean and sharp bandsHigh membrane binding affinityHigh storage stabilityBio Basic prestained protein ladder introductionBio Basic prestained protein ladder is designed for estimating the approximate size of separated proteins on SDS-PAGE gel. The ladders are a mixture of recombinant proteins ranging from 10 kD to 250 kD. Red or green bands at 70 kD and 25 kD provide easy references for molecular weight identification. The molecular weights of the prestained ladders are confirmed using the Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. The protein ladders are highly stable with minimal band broadening during storage. Products are conveniently packaged and ready to use， with no heating, diluting or additional reducing agent required.Bio Basic Prestained protein ladder has high size precisionTo perform molecular weight (MW) size precision evaluation, both unstained and prestained ladders were resolved on a 4-15% gradient SDS-PAGE Gel (BioRad #4561083) followed by Coomassie BB staining (left panel). MWs of all protein bands were indicated at their migration positions (right panel). As seen in lane 3, 4 and 5, some prestained protein bands show significant MWs inconsistencies between BioRad’s and Thermo’s products. Similar inconsistent bands are also observed between two ladders from the same manufacturer (Thermo #26616 and #26619). Generally, unstained protein ladders are considered to be more precise. Here, two unstained ladders (Thermo #26630 and NEB #7703, a third-party’s product) were included as standard references (lane 1&2). When compared with unstained ladders, there are obvious imprecise prestained bands in both popular brands’ products. Interestingly, the MWs of Thermo’s prestained ladders (#26616 and #26619) do not match their own unstained protein ladder (#26630). There are no significant imprecise bands in the Bio Basic ladder (lane 6). It should be noted that presteined ladders were originally designed for approximative MW reference and therefore, neither represent an absolute standard.*Normally, all prestained protein ladders have lot-to-lot variation. This evaluation only represented the tested lot and did not intend to conclude a judgement of all other lots of listed brands’ products.**The evaluation was performed on Tris-Glycine buffer system. The mobility of prestained proteins can vary in different SDS-PAGE buffer systems.
Broad range (10-250 kD)Dual-Color or Tri-ColorHigh size precision and lot consistencyClean and sharp bandsHigh membrane binding affinityHigh storage stabilityBio Basic prestained protein ladder introductionBio Basic prestained protein ladder is designed for estimating the approximate size of separated proteins on SDS-PAGE gel. The ladders are a mixture of recombinant proteins ranging from 10 kD to 250 kD. Red or green bands at 70 kD and 25 kD provide easy references for molecular weight identification. The molecular weights of the prestained ladders are confirmed using the Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. The protein ladders are highly stable with minimal band broadening during storage. Products are conveniently packaged and ready to use， with no heating, diluting or additional reducing agent required.Bio Basic Prestained protein ladder has high size precisionTo perform molecular weight (MW) size precision evaluation, both unstained and prestained ladders were resolved on a 4-15% gradient SDS-PAGE Gel (BioRad #4561083) followed by Coomassie BB staining (left panel). MWs of all protein bands were indicated at their migration positions (right panel). As seen in lane 3, 4 and 5, some prestained protein bands show significant MWs inconsistencies between BioRad’s and Thermo’s products. Similar inconsistent bands are also observed between two ladders from the same manufacturer (Thermo #26616 and #26619). Generally, unstained protein ladders are considered to be more precise. Here, two unstained ladders (Thermo #26630 and NEB #7703, a third-party’s product) were included as standard references (lane 1&2). When compared with unstained ladders, there are obvious imprecise prestained bands in both popular brands’ products. Interestingly, the MWs of Thermo’s prestained ladders (#26616 and #26619) do not match their own unstained protein ladder (#26630). There are no significant imprecise bands in the Bio Basic ladder (lane 6). It should be noted that presteined ladders were originally designed for approximative MW reference and therefore, neither represent an absolute standard.*Normally, all prestained protein ladders have lot-to-lot variation. This evaluation only represented the tested lot and did not intend to conclude a judgement of all other lots of listed brands’ products.**The evaluation was performed on Tris-Glycine buffer system. The mobility of prestained proteins can vary in different SDS-PAGE buffer systems.Total Product Size:100ul
Individual Container Size:100ul
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41105337
UNSPSC Category:Protein Molecular Weight Markers
BioBasic无论您需要针对实验室高度专业化的Western Blot或免疫组织化学，ELISA还是雄心勃勃的项目的抗体，Bio Basic都能帮助您获得所需的结果。我们的抗体合成软件包结构合理，可让您自由（从cDNA，肽或蛋白质）合成抗体，该抗体将在基本应用中成功或保证在您选择的测定中起作用。我们合格的科学家团队将协助您选择适合您项目目标的最佳包装，并在整个防毒剂生产过程中向您提供最新信息。 
