生物测定系统/QuantiChrom™葡萄糖脱氢酶检测试剂盒/100次检测/DGDH-100

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¥6580.00
货号:DGDH-100
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品牌:BioAssay Systems
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商品描述

QuantiChrom™ Glucose Dehydrogenase Assay Kit

QuantiChrom™ Glucose Dehydrogenase Assay Kit Catalog No: DGDH-100
Price: $329 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: RT Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: RT
Glucose Dehydrogenase Assay Kit
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  • Assay Service
ProtocolSDS

Application

  • For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 200 U/L for 15 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD565nm

Samples

  • Biological samples (e.g. plasma, serum, tissue and culture media).

Species

  • All

Size

  • 100 tests

Detection Limit

  • 0.5 U/L

Shelf Life

  • 6 months

More Details

  • GLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway. BioAssay Systems' non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems酸性磷酸酶(ACP)是一种在消化过程中催化磷酸基团从其他分子上裂解的酶。酸性磷酸酶可以在溶酶体中发现,并在与内体融合,酸化pH值后变得有活性,从而为ACP创造了最佳环境。ACP还可以在骨骼,脾脏,肝脏,肾脏和血液中发现。血清水平可以用作前列腺癌的生物标志物,尽管前列腺特异性抗原(PSA)被更广泛地使用。BioAssay Systems非放射性比色ACP分析基于从合成底物上裂解对硝基苯酚。加入终止剂后,对硝基苯酚会显色。加入终止剂后在405 nm处吸光度的增加与酶活性成正比。