生物测定系统/酶染色与贸易;柠檬酸盐检测试剂盒/100次检测/ECIT-100

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¥6780.00
货号:ECIT-100
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品牌:BioAssay Systems
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商品描述

EnzyChrom™ Citrate Assay Kit

EnzyChrom™ Citrate Assay Kit Catalog No: ECIT-100
Price: $339 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: On Ice Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
Citrate Assay Kit
  • Product Overview
  • Product FAQ
  • Product Citations
  • Assay Service
ProtocolSDS

Application

  • For quantitative determination of citrate or citric acid and evaluation of drug effects on citrate metabolism.

Key Features

  • Fast and sensitive. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD570nm, or FL530/585nm

Samples

  • Serum, plasma, agriculture etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • OD, FL: 4, 0.5 μM.

Shelf Life

  • 6 months

More Details

  • CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. BioAssay Systems' Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Fu, Xuekun, et al (2018). Runx2/Osterix and zinc uptake synergize to orchestrate osteogenic differentiation and citrate containing bone apatite formation. Advanced Science 5.4: 1700755. Assay: Citrate in mice cells. Trivedi, Amit Kumar, et al. (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 184): 34-40. Assay: Citrate in bird plasma.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems酸性磷酸酶(ACP)是一种在消化过程中催化磷酸基团从其他分子上裂解的酶。酸性磷酸酶可以在溶酶体中发现,并在与内体融合,酸化pH值后变得有活性,从而为ACP创造了最佳环境。ACP还可以在骨骼,脾脏,肝脏,肾脏和血液中发现。血清水平可以用作前列腺癌的生物标志物,尽管前列腺特异性抗原(PSA)被更广泛地使用。BioAssay Systems非放射性比色ACP分析基于从合成底物上裂解对硝基苯酚。加入终止剂后,对硝基苯酚会显色。加入终止剂后在405 nm处吸光度的增加与酶活性成正比。