生物测定系统/QuantiChrom™GTPase检测试剂盒/200测试/DATG-200

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¥6980.00
货号:DATG-200
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品牌:BioAssay Systems
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商品描述

QuantiChrom™ GTPase Assay Kit

QuantiChrom™ GTPase Assay Kit Catalog No: DATG-200
Price: $349 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: RT Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: 4°C
GTPase Assay Kit
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ProtocolSDS

Application

  • For quantitative determination of ATPase, GTPase or any enzyme activity that liberated free phosphate and high-throughput screen for their inhibitors.

Key Features

  • High sensitivity: detection of 0.007 U/L ATPase or GTPase activity.
  • Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes.
  • Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method

  • OD620nm

Samples

  • ATPase and GTPase

Species

  • All

Size

  • 200 tests

Detection Limit

  • 0.007 U/L

Shelf Life

  • 12 months

More Details

  • ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation. BioAssay Systems QuantiChrom™ ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems酸性磷酸酶(ACP)是一种在消化过程中催化磷酸基团从其他分子上裂解的酶。酸性磷酸酶可以在溶酶体中发现,并在与内体融合,酸化pH值后变得有活性,从而为ACP创造了最佳环境。ACP还可以在骨骼,脾脏,肝脏,肾脏和血液中发现。血清水平可以用作前列腺癌的生物标志物,尽管前列腺特异性抗原(PSA)被更广泛地使用。BioAssay Systems非放射性比色ACP分析基于从合成底物上裂解对硝基苯酚。加入终止剂后,对硝基苯酚会显色。加入终止剂后在405 nm处吸光度的增加与酶活性成正比。