生物测定系统/EnzyChrom™谷胱甘肽过氧化物酶检测试剂盒/100试验/EGPX-100

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¥5580.00
货号:EGPX-100
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EnzyChrom™ Glutathione Peroxidase Assay Kit

EnzyChrom™ Glutathione Peroxidase Assay Kit Catalog No: EGPX-100
Price: $279 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: On Ice Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
Glutathione Peroxidase Assay Kit
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ProtocolSDS

Application

  • For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity.

Key Features

  • Sensitive and accurate. Use 10 μL sample. Linear detection range 40 to 800 U/L GPX activity.

Method

  • OD340nm

Samples

  • Biological

Species

  • All

Size

  • 100 tests

Detection Limit

  • 40 U/L

Shelf Life

  • 6 months

More Details

  • Glutathione peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR), Simple, direct and high-throughput assays for GPX activity find wide applications. BioAssay Systems improved assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
De Maistre, S., Vallee, N., Gaillard, S., Duchamp, C., & Blatteau, J. E. (2018). Stimulating fermentation by the prolonged acceleration of gut transit protects against decompression sickness. Scientific reports, 8(1), 10128. Assay: Glutahione peroxidase in rat plasma. Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: Glutahione peroxidase in D. melanogaster tissues.Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Glutahione peroxidase in rat tissues. Kook, S. H., Cheon, S. R., Kim, J. H., Choi, K. C., Kim, M. K., & Lee, J. C. (2017). Dietary hydroxycinnamates prevent oxidative damages to liver, spleen, and bone marrow cells in irradiation-exposed mice. Food science and biotechnology, 26(1), 279-285. Assay: Glutahione peroxidase in mice tissues. Adeyemi, K. D., Ismail, M., Ebrahimi, M., Sabow, A. B., Shittu, R. M., Karim, R., & Sazili, A. Q. (2016). Fatty acids, lipid and protein oxidation, metmyoglobin reducing activity and sensory attributes of biceps femoris muscle in goats fed a canola and palm oil blend. South African Journal of Animal Science, 46(2), 139-151. Assay: Glutahione peroxidase in goat tissues.Adeyemi, K. D., Sabow, A. B., Aghwan, Z. A., Ebrahimi, M., Samsudin, A. A., Alimon, A. R., & Sazili, A. Q. (2016). Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend. Journal of animal science and technology, 58(1), 6. Assay: Glutahione peroxidase in goat serum. Adeyemi, K. D., Shittu, R. M., Sabow, A. B., Abubakar, A. A., Karim, R., Karsani, S. A., & Sazili, A. Q. (2016). Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of animal science and technology, 58(1), 23. Assay: Glutahione peroxidase in boer tissues. Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutahione peroxidase in fish plasma. Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutathione Peroxidase in fish plasma.Ahmed, MA et al (2014). Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sciences 110(2): 61-9. Assay: Glutahione peroxidase in rat brain tissue. Chen, W et al (2014). Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats. PLoS One 9(2):e88076. Assay: Glutahione peroxidase in rat hippocampus tissue. Effendy, NM and Shuid, AN (2014). Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis rat Model. Nutrients 6(8): 3288-302. Assay: Glutahione peroxidase in rat bone tissue. Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Toxicological & Environmental Chemistry 96(2): 307-317. Assay: Glutahione peroxidase in rat.Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione peroxidase in deer blood. Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione Peroxdiase in Deer Blood.Calamita G et al (2012). Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol. Biol Cell 104(6):342-51. Assay: Glutahione peroxidase in human blood. Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Glutathione Peroxidase in human blood. To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems酸性磷酸酶(ACP)是一种在消化过程中催化磷酸基团从其他分子上裂解的酶。酸性磷酸酶可以在溶酶体中发现,并在与内体融合,酸化pH值后变得有活性,从而为ACP创造了最佳环境。ACP还可以在骨骼,脾脏,肝脏,肾脏和血液中发现。血清水平可以用作前列腺癌的生物标志物,尽管前列腺特异性抗原(PSA)被更广泛地使用。BioAssay Systems非放射性比色ACP分析基于从合成底物上裂解对硝基苯酚。加入终止剂后,对硝基苯酚会显色。加入终止剂后在405 nm处吸光度的增加与酶活性成正比。