asuragen/AmplideX®PCR/CE C9orf72试剂盒/50/49581

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商品描述

AmplideX® PCR/CE C9orf72 Kit

C9orf72 main web image smallThe AmplideX PCR/CE C9orf72 Kit (RUO) is a research product for the detection of GGGGCC repeats in the C9orf72 gene. These reagents provide a single-tube PCR approach based on a Repeat-Primed PCR (RP-PCR) design to profile repeat sequences in the C9orf72 gene.

Features & Benefits

Asuragen has established the market-leading technology for the reliable amplification and analysis of CGG triplet repeats in the FMR1 gene. The AmplideX PCR/CE C9orf72 Kit (RUO) is an expansion of this technology into the neurogenetics space. The kit provides clinical and pharmaceutical researchers with a reliable and reproducible method for high-resolution genotyping of hexanucleotide repeat expansions in the C9orf72 gene, which is of increasing interest in frontotemporal dementia (FTD) – the second most common form of early onset dementia after Alzheimer’s Disease – and amyotrophic lateral sclerosis (ALS).

Reduced ComplexityAnalysis of the C9orf72 gene has been simplified through:

  • Implementation of proprietary 2-in-1 PCR solution for amplifying GC-rich regions
  • A single sourced kit consisting of all PCR reagents needed for C9orf72 repeat amplification
  • Streamlined workflow with minimal hands-on time

Optimized WorkflowValuable operator hands-on time has been significantly reduced through:

  • Single PCR reaction for both sizing and screening
  • Direct injection of PCR products (no PCR clean up) to Capillary Electrophoresis platforms
  • Decreased need for Southern blot analysis

Quality PerformancePerforming C9orf72 analysis with greater sensitivity and accuracy:

  • Five-fold improvement in length of alleles sized: accurate sizing up to 145 repeats
  • Detection of alleles greater than 145 repeats
  • Reveal low-level mosaicism and minor alleles

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*For Research Use Only. Not for use in Diagnostic procedures.

Analytical Characteristics

Analytical characteristics of AmplideX PCR/CE C9orf72 Kit:

  • Accurately sizes up to 145 repeats (Figure 1)
  • Detects alleles >145 repeats (Figure 2)
  • Resolves zygosity (Figure 3)

Figure 1: Coriell cell line ND06769: The AmplideX Kit correctly sizes a primary allele at 13 repeats and a minor allele at 44 repeats. The expanded allele is also detected in the electropherogram.

C9orf72 web Figure 1 combined blue

Figure 2. Coriell cell line ND06769: Alleles up to 145 repeats can be detected above background and correctly sized, alleles above 145 repeats are amplified and detected as expanded.

C9orf72 Web Figure 2 combined with Inset

Figure 3: Side by side comparison of 2 Coriell cell lines: ND08619 (7,7 homozygous) and ND12102 (7,expanded heterozygous): Extension of the repeat-primed peak pattern beyond the shorter allele clearly differentiates the heterozygous sample from homozygous sample.

C9orf72 Web Figure 3 combined blue

Ordering

Product NameNumber of ReactionsCatalog Number
AmplideX® PCR/CE C9orf72 Kit (RUO)5049581

T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com

View Sales Contacts

AmplideX® PCR/CE C9orf72 Kit

C9orf72 main web image smallThe AmplideX PCR/CE C9orf72 Kit* is a research product for the detection of GGGGCC repeats in the C9orf72 gene. These reagents provide a single-tube PCR approach based on a Repeat-Primed PCR (RP-PCR) design to profile repeat sequences in the C9orf72 gene.

Features & Benefits

Asuragen has established the market-leading technology for the reliable amplification and analysis of CGG triplet repeats in the FMR1 gene. The AmplideX PCR/CE C9orf72 Kit (RUO) is an expansion of this technology into the neurogenetics space. The kit provides clinical and pharmaceutical researchers with a reliable and reproducible method for high-resolution genotyping of hexanucleotide repeat expansions in the C9orf72 gene, which is of increasing interest in frontotemporal dementia (FTD) – the second most common form of early onset dementia after Alzheimer’s Disease – and amyotrophic lateral sclerosis (ALS).

Reduced ComplexityAnalysis of the C9orf72 gene has been simplified through:

  • Implementation of proprietary 2-in-1 PCR solution for amplifying GC-rich regions
  • A single sourced kit consisting of all PCR reagents needed for C9orf72 repeat amplification
  • Streamlined workflow with minimal hands-on time

Optimized WorkflowValuable operator hands-on time has been significantly reduced through:

  • Single PCR reaction for both sizing and screening
  • Direct injection of PCR products (no PCR clean up) to Capillary Electrophoresis platforms
  • Decreased need for Southern blot analysis

Quality PerformancePerforming C9orf72 analysis with greater sensitivity and accuracy:

  • Five-fold improvement in length of alleles sized: accurate sizing up to 145 repeats
  • Detection of alleles greater than 145 repeats
  • Reveal low-level mosaicism and minor alleles

Analytical Characteristics

Analytical characteristics of AmplideX PCR/CE C9orf72 Kit:

  • Accurately sizes up to 145 repeats (Figure 1)
  • Detects alleles >145 repeats (Figure 2)
  • Resolves zygosity (Figure 3)

Figure 1: Coriell cell line ND06769: The AmplideX Kit correctly sizes a primary allele at 13 repeats and a minor allele at 44 repeats. The expanded allele is also detected in the electropherogram.

C9orf72 web Figure 1 combined blue

Figure 2. Coriell cell line ND06769: Alleles up to 145 repeats can be detected above background and correctly sized, alleles above 145 repeats are amplified and detected as expanded.

C9orf72 Web Figure 2 combined with Inset

Figure 3: Side by side comparison of 2 Coriell cell lines: ND08619 (7,7 homozygous) and ND12102 (7,expanded heterozygous): Extension of the repeat-primed peak pattern beyond the shorter allele clearly differentiates the heterozygous sample from homozygous sample.

C9orf72 Web Figure 3 combined blue

Ordering

Product NameNumber of ReactionsCatalog Number
AmplideX® PCR/CE C9orf72 Kit (RUO)5049581

T 1-877-777-1874; 512-681-5200 F 1-512-681-5202 E orders@asuragen.com

View Sales Contacts
asuragen的AmplideX®PCR / CE HTT试剂盒 AmplideX PCR / CE  HTT 试剂盒*使HTT  基因中CAG三核苷酸重复序列的检测和确定  比以往更加容易。只需一次PCR反应,该测定法即可克服相邻SNP的挑战,可变的CCG重复序列可准确可靠地报告一天中重复序列的数量,从10ng gDNA即可。  特点与优势分析特性订购信息特点与优势AmplideX PCR / CE HTT试剂盒包含用于对HTT CAG重复区域进行两引物,锚定引物PCR的试剂,然后通过毛细管电泳确定片段大小。这种单管PCR反应提供了简化的工作流程,使用现成的实验室设备可以在大约6小时内得到样品。降低复杂度专有的PCR解决方案,用于富含GC的扩增和检测消除了对多个PCR的需求–一个结果,简单的分析解决接合性并检测大扩展优化的工作流程配套齐全的端到端解决方案,可大大减少动手时间一次转换即可实现样品到结果与AmplideX PCR / CE DMPK试剂盒相同的PCR和CE条件质量结果可靠,明确的结果和稳定的峰峰值模式在整个CAG重复范围内准确调整大小软件将原始碱基对数据转换为重复数