Features & Benefits

The QuantideX NGS DNA Hotspot 21 Kit combines Asuragen’s unique NGS-in-a-Box™ solution with a streamlined testing workflow to enable unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE samples.

Reduced ComplexityAssay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration

• Detects >1,500 variants from commonly mutated genomic regions across multiple tumor types
• End-to-end kitted solution
• Fully integrated data analysis pipeline

Optimized WorkflowProvides operational efficiencies to reduce testing costs, hands-on and total turnaround time

• Reduced labor required for library preparation
• Improved turnaround time enables higher throughput
• Common workflow across portfolio streamlines training & implementation

Quality PerformanceHighly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures

• Highly sensitive detection of DNA-based variants
• Low input (~20ng) of DNA from FFPE
• Sample-Aware™ bioinformatics analysis and sample quality control

Product Description

Relevant ContentThe QuantideX NGS DNA Hotspot 21 Kit detects over 1,500 genomic variants as reported in the Catalogue of Somatic Mutations in Cancer (COSMIC) Database within 21 genes, representing approximately 80% of known variants within these targets.

Table 1: Mutation coverage of QuantideX NGS DNA Hotspot 21 Kit

Table 2: Clinical relevance of covered mutations

¹NCCN Clinical Practice Guidelines*Includes gastric, pancreatic, glicomas, sarcomas, and other tumor types

A Fully Integrated WorkflowA unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to use.

Includes:

• An internal quality control kit to measure the absolute copy number of amplifiable DNA and reports PCR inhibition
• Gene-specific PCR primers and Master Mix reagents
• Dual-index barcodes for sample multiplexing
• Library purification and quantification reagents
• Integrated data analysis and reporting software (QuantideX NGS Reporter)

Rapid & EfficientAdopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.

• Fully integrated workflow reduces complexity and ensures reliable reagent quality
• Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
• Integrated kit reduces overall workflow time

Performance Data

Highly Sensitive and Accurate Detection of DNA Mutations

QuantideX® NGS Reporter

Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.

Operates on a standard desktop computer – Install locally on a desktop computer using Windows^® operating system.  No prior bioinformatics experience or large server environments required.

Comprehensive– Full bioinformatics and reporting of variants (SNVs, Indels), and standard QC metrics are automatically calculated.

Integrates Sample-Aware™ – Bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.

Resources

Videos

Posters

Analytical Validation Of The QuantideX^® NGS DNA Hotspot 21 Kit, A Diagnostic NGS System for the Detection of Actionable Mutations in FFPE TumorsView full poster

A Machine-Learning Framework for Accurate Classification and Quantification of Oncogenic Variants Using the QuantideX^® NGS DNA Hotspot 21 KitView full poster

A Simple and Versatile Next-Generation Sequencing Technology for Co-Detection of RNA Structural Variants and DNA Mutations in Lung CancerView full poster 

Features & Benefits

The QuantideX NGS DNA Hotspot 21 Kit combines Asuragen’s unique NGS-in-a-Box™ solution with a streamlined testing workflow to enable unprecedented NGS workflow efficiency and high sensitivity at low input amounts from precious FFPE samples.

Reduced ComplexityAssay incorporates sample-to-data solutions in a unique NGS-in-a-Box™ configuration

• Detects >1,500 variants from commonly mutated genomic regions across multiple tumor types
• End-to-end kitted solution
• Fully integrated data analysis pipeline

Optimized WorkflowProvides operational efficiencies to reduce testing costs, hands-on and total turnaround time

• Reduced labor required for library preparation
• Improved turnaround time enables higher throughput
• Common workflow across portfolio streamlines training & implementation

Quality PerformanceHighly sensitive assay with integrated, Sample-Aware™ bioinformatics software and built-in quality checks to minimize erroneous results and sample failures

• Highly sensitive detection of DNA-based variants
• Low input (~20ng) of DNA from FFPE
• Sample-Aware™ bioinformatics analysis and sample quality control

Product Description

Relevant ContentThe QuantideX NGS DNA Hotspot 21 Kit detects over 1,500 genomic variants as reported in the Catalogue of Somatic Mutations in Cancer (COSMIC) Database within 21 genes, representing approximately 80% of known variants within these targets.  Several of these variants are associated with approved therapies, while others are currently in trials to clarify their clinical significance.

Table 1: Mutation coverage of QuantideX NGS DNA Hotspot 21 Kit

Table 2: Clinical relevance of covered mutations

¹NCCN Clinical Practice Guidelines*Includes gastric, pancreatic, glicomas, sarcomas, and other tumor types

A Fully Integrated WorkflowA unique NGS-in-a-Box™ configuration offers a simplified and fully integrated NGS workflow with cGMP-manufactured reagents, components and controls ready to use.

Includes:

• An internal quality control kit to measure the absolute copy number of amplifiable DNA and reports PCR inhibition
• Gene-specific PCR primers and Master Mix reagents
• Dual-index barcodes for sample multiplexing
• Library purification and quantification reagents
• Integrated data analysis and reporting software (QuantideX NGS Reporter)

Rapid & EfficientAdopt and run NGS-based analysis with minimum investment of time and resources, regardless of NGS experience and infrastructure.

• Fully integrated workflow reduces complexity and ensures reliable reagent quality
• Push-button analysis & reporting makes bioinformatics easy and efficient, regardless of experience level
• Integrated kit reduces overall workflow time

Performance Data

Highly Sensitive and Accurate Detection of DNA Mutations

QuantideX® NGS Reporter

Push-button analytics & reporting suite – Set-up-and-go workflow designed for easy installation and implementation, right out of the box.

Operates on a standard desktop computer – Install locally on a desktop computer using Windows^® operating system.  No prior bioinformatics experience or large server environments required.

Comprehensive – Full bioinformatics and reporting of variants (SNVs, Indels) and standard QC metrics are automatically calculated.

Integrates Sample-Aware™ – bioinformatics with integrated functional template copy number analysis dramatically reduces false-call rates.

Resources

Videos

Posters

Analytical Validation Of The QuantideX^® NGS DNA Hotspot 21 Kit, A Diagnostic NGS System for the Detection of Actionable Mutations in FFPE TumorsView full poster

A Machine-Learning Framework for Accurate Classification and Quantification of Oncogenic Variants Using the QuantideX^® NGS DNA Hotspot 21 KitView full poster

A Simple and Versatile Next-Generation Sequencing Technology for Co-Detection of RNA Structural Variants and DNA Mutations in Lung CancerView full poster