| Brilliant Blue Gused for protein staining in SDS-PAGE, Blue Native PAGE, and the Bradford Method; selective inhibitor of the P2X purinoceptor channel P2X7 |
Sample solution is provided at 25 µL, 10mM.
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- Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
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| Cas No. | 6104-58-1 | SDF | Download SDF |
| Synonyms | Acid Blue 90,CBBG,Coomassie Brilliant Blue G-250,NSC 328382 | ||
| Chemical Name | N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium, sodium salt | ||
| Canonical SMILES | CC1=CC(N(CC)CC2=CC=CC(S(=O)([O-])=O)=C2)=CC=C1/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C=C/C(C=C5C)=[N+](CC6=CC=CC(S(=O)([O-])=O)=C6)/CC.[Na+] | ||
| Formula | C47H48N3O7S2 • Na | M.Wt | 854 |
| Solubility | ≥21.35mg/mL in DMSO | Storage | Store at RT |
| Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Brilliant Blue G is also a selective inhibitor of the P2X purinoceptor channel P2X7 [1].
P2X receptors are membrane ion channels activated in response to the binding of extracellular ATP. Seven P2X subtypes have been identified. P2X receptors have been involved in in diverse patho- and physiological processes, such as the autonomic nervous system, afferent signalling, chronic pain, and in autocrine loops of endothelial and epithelial cells. The P2X7 receptor plays a prominent role in certain neurologic disorders, such as ischemia-reperfusion injury, Alzheimer"s disease, spinal cord injury and sensory neuropathies [2].
In HEK293 cells heterologously expressing human P2X7 receptors, Brilliant Blue G noncompetitively inhibited rat and human P2X7 receptors with IC50 values of 10 and 200 nM, respectively. The IC50 values for inhibition of the other P2X receptors ranged from 2 to >30 μM; Brilliant Blue G inhibited the rat and human P2X4 receptors with the IC50 values of >10 and 3.2 μM [1]. Subretinal injection of BBG caused retinal cell degeneration at lower concentrations. Subretinal injection of BBG (0.25 mg/mL) provided satisfactory biocompatibility [3].
References:[1] Jiang L H, Mackenzie A B, North R A, et al. Brilliant blue G selectively blocks ATP-gated rat P2X7 receptors[J]. Molecular pharmacology, 2000, 58(1): 82-88.[2] Sperlágh B, Vizi E S, Wirkner K, et al. P2X 7 receptors in the nervous system[J]. Progress in neurobiology, 2006, 78(6): 327-346.[3] Ueno A, Hisatomi T, Enaida H, et al. Biocompatibility of brilliant blue G in a rat model of subretinal injection[J]. Retina, 2007, 27(4): 499-504.
