| Bithionolpotent inhibitor of soluble adenylyl cyclase (sAC) |
Sample solution is provided at 25 µL, 10mM.
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- Purity = 98.00%
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| Cas No. | 97-18-7 | SDF | Download SDF |
| Chemical Name | 6,6"-thiobis(2,4-dichlorophenol) | ||
| Canonical SMILES | ClC1=CC(Cl)=C(O)C(SC2=CC(Cl)=CC(Cl)=C2O)=C1 | ||
| Formula | C12H6Cl4O2S | M.Wt | 356.05 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
IC50: 19 μM – 60 μM for various ovarian cancer cell lines
Bithionol is a potent inhibitor of soluble adenylyl cyclase (sAC).
Adenylyl cyclase is an enzyme with critical regulatory roles in cells. All classes of adenylyl cyclases can catalyse the conversion of adenosine triphosphate to 3",5"-cyclic AMP and pyrophosphate.
In vitro: Bithionol could cause dose-dependent cytotoxicity toward all tested ovarian cancer cell lines regardless of their sensitivities to cisplatin. Moreover, such cell death appeared to be via caspases mediated apoptosis. In addition, the mechanism of action appeared to be partially by cell cycle arrest, ROS generation as well as ATX inhibition [1].
In vivo: Oral toxicity of bithionol sulfoxide was assessed in acute toxicity studies in mice and rats. The median lethal dose (LD50) values in mice were > 1000 mg/kg and < 5000="" mg/kg="" after="" 21="" days;="" the="" male="" rat="" ld50="" value="" was="" around="" 5000="" mg/kg="" after="" 48="" h.="" moreover,="" the="" hepatic="" toxicity="" was="" observed="" at="" all="" tested="" doses.="" increases="" in="" serum="" ast="" were="" observed="" with="" the="" high="" doses,="" suggesting="" that="" mitochondria="" were="" affected.="" in="" addition,="" the="" histological="" study="" indicated="" more="" intense="" periportal="" fatty="" infiltration="" with="" high="" doses="" at="" 5000="" and="" 500="" mg/kg="" [2].="">
Clinical trial: In a previous study, bithionol was orally given to patients with acute fascioliasis at the daily dose of 25 mg/kg for 10 days. Results showed that all patients were cured. The follow-up period after the first course of treatment was between 16 and 47 months and no major side effects were found [3].
References:[1] Ayyagari VN,Brard L. Bithionol inhibits ovarian cancer cell growth in vitro - studies on mechanism(s) of action. BMC Cancer.2014 Feb 4;14:61. [2] Lavric A, Skubic V, Senk L, Lukanc G, Kacl E. Oral toxicity of bithionol sulfoxide in mice and rats. Zbornik Veterinarske Fakultete Univerza Ljubljana 1990 27(1): 33-39[3] Bacq Y,Besnier JM,Duong TH,Pavie G,Metman EH,Choutet P. Successful treatment of acute fascioliasis with bithionol. Hepatology.1991 Dec;14(6):1066-9.
