Make micropipettes to record cellular concentrations
- 0.1 mL
WPI’s Liquid Ion Exchangers (LIX), for use with the FD223A Electrometer, allow intracellular measurements to be made for cations (hydrogen, potassium and calcium).
Liquid Ion Exchanger Instruction Manual
| ION | H+ | K+ | Ca++ |
| CATALOG NO. | IE010 | IE190 | IE200 |
| SELECTIVITY COEFFICIENTS* | |||
| Na+ | 12.7 | 1.97 | 5.5 |
| Mg++ | — | 2.95 | 4.9 |
| K+ | — | — | 5.4 |
| Ca++ | — | 2.7 | — |
| USEFUL pH RANGE | 2-10 | 4-10 | 4-10 |
| SLOPE | 56 mV | 58 mV | 28 mV |
| LINEAR RANGE | pH 4-12 | pK 0-3 | pCa 1-7 |
| APPROX. EQUIV. | — | Corning477317 | ETH1001 |
| *Selectivity Coefficients are expressed here as -log Kij or pKij. | |||
Yoshida, T., Nin, F., Ogata, G., Uetsuka, S., Kitahara, T., Inohara, H., … Hibino, H. (2015). NKCCs in the fibrocytes of the spiral ligament are silent on the unidirectional K+ transport that controls the electrochemical properties in the mammalian cochlea. Pflügers Archiv - European Journal of Physiology, 467(7), 1577–1589. https://doi.org/10.1007/s00424-014-1597-9
Kuwana, S., Okada, Y., & Natsui, T. (1998). Effects of extracellular calcium and magnesium on central respiratory control in the brainstem-spinal cord of neonatal rat. Brain Research, 786(1–2), 194–204. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/9555011
Welsh, D. G., Jackson, W. F., & Segal, S. S. (1998). Oxygen induces electromechanical coupling in arteriolar smooth muscle cells: a role for L-type Ca 2+ channels. American Journal of Physiology-Heart and Circulatory Physiology, 274(6), H2018–H2024. https://doi.org/10.1152/ajpheart.1998.274.6.H2018
