Direct quantitative measurements of hydrogen peroxide in biological samples
- Gas permeable polymer membrane sleeve blocks liquids, ions and particulate matter
- Incorporated reference electrode
- For use with Apollo1000, Apollo4000, TBR4100 and TBR1025
- Package of 1
See the latest Biosensor Data Sheet.
Benefits
- Response Time: < 5 s (90%)
- Detection Limit: < 100 nM to 100 μM (100 mM custom)
- Drift: < 0.2 pA/min
- Sensitivity: 8 pA/μM
Applications
- Cell Cultures
NOTE: The 2mm biosensors come with a spare membrane sleeve, 1 cc syringe, an MF28G67 MicroFil needle and 10 mL of filling solution.
Despite the recognized importance of this oxidant in biology, real-time measurements at low concentration have been difficult. The hydrogen peroxide sensors developed by WPI are designed to compliment existing high sensitivity fluorescent approaches with direct quantitative measurement in biological samples in the low nM range.
The ISO-HPO-2 is a 2.0 mm stainless steel sensor, with replaceable membrane sleeves (600012) and an internal refillable electrolyte (100042). It is designed for use in cell cultures and similar applications.
The ISO-HPO-100 is a 100 µm tip diameter hydrogen peroxide micro sensor designed for use in tissues and similar applications. The design is based on a platinum wire sensing electrode coated with a proprietary membrane to enhanceH2O2 detection.
These sensors incorporate WPI’s proprietary combination electrode technology whereby the hydrogen peroxide sensing element and separate reference electrode are encased within a single Faraday-shielded probe design. This design has been shown to enhance performance during measurements and minimizes overall sensor size.
Our hydrogen peroxide (H2O2) sensors work with the TBR4100 and TBR1025 free radical analyzers.
Hydrogen peroxide in biological systems
Hydrogen Peroxide is produced in biological systems by controlled pathways at low concentrations that impact on cell signaling. At higher concentrations inflammatory cells produce local intense amounts of this oxidant to kill pathogens. In the progress of human disease, uncontrolled formation of hydrogen peroxide from the mitochondrial respiratory chain and enzymes, such as xanthine oxidase, can occur (Prof. Victor Darley-Usmar, Univ. of Alabama, personal communication).
ISO-HPO-2 Instruction Manual
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Universal Dipping Probe Stand42200For pricing, Customers outside of the US and Canada, please contact your distributor.
