Product Features:

Recombinant Mouse IFN Beta without carrier protein
Less than 1 EU/μg endotoxin
Suitable for in vivo injection studies

Catalog No.	Pack Size	Price	Quantity
12401-1	1 x 10⁵ units	$380.00

Specifications
Tech Info / Data
Citations
Documentation

Specifications

Formulation	Supplied frozen in 20 mM HEPES, pH 6.0, 0.5M NaCl; 6% Glycerol
Molecular Weight	19.6 kDa
Source	Gene was obtained from mouse DNA expressed in E. coli modified as described in Day, et al. (1992)"Engineered disulfide bond greatly increases specific activity of recombinant murine interferon beta" ( J. Interferon Res. 12: 139-43)
Purity	> 95% by SDS-PAGE stained by Coomassie BlueEndotoxin level < 1 EU/μg
Bioactivity	Measured using a cytopathic effect inhibition assay on Mouse (L929) cells with EMCV. EC₅₀ for IFNβ is ~ 2.5 U/ml
Storage	For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles
Synonyms	Mouse Beta IFN, Mouse Beta Interferon, Mouse IFN Beta, Mouse IFNB, Mouse Type I IFN beta, Mouse Fibroblast IFN, Mouse Fibroblast Interferon, Mouse Type I Interferon Beta

Tech Info / Data

Background:

E.coli expressed recombinant Mouse IFN Beta protein supplied in carrier-free formulation to minimize possible immnogenic reaction by host animal in in vivo studies

Citations

22 Citations:

Grunewald, Matthew, et al. (2019). The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression. PLOS Pathogens, 24 pgs. PMID: 31095648. (link)

Wang, A, et al. (2019). Specific sequences of infectious challenge lead to secondary hemophagocytic lymphohistiocytosis-like disease in mice. PNAS. PMID: 30674681. (link)

Li, S, et al. (2018). The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING. Cell Reports, 25 pgs. PMID: 30566866. (link)

Cui, Huachun, et al. (2018). Interferon regulatory factor 2 inhibits expression of glycolytic genes and lipopolysaccharide induced pro-inflammatory responses in macrophages. Journal of Immunology. PMID: 29563175. (link)

McDonough, Ashley, et al. (2017). Ischemia/Reperfusion Induces Interferon-Stimulated Gene Expression in Microglia. The Journal of Neuroscience, 17 pgs. PMID: 28747383. (link)

Stier, Matthew, et al. (2017). STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection. Journal of Immunology. PMID: 28576981. (link)

Benci, Joseph, et al. (2016). Tumor Interferon Signaling Regulates a Multigenic Resistance Program to Immune Checkpoint Blockade. CellPress, 28 pgs. PMID: 27912061. (link)

Labzin,Larisa, et al. (2015). ATF3 Is a Key Regulator of Macrophage IFN Responses. Journal of Immunology, 11 pgs. PMID: 26416280. (link)

Majumdar, Tanmay, et al. (2015). Induction of Interferon-Stimulated Genes by IRF3 Promotes Replication of Toxoplasma gondii. PLOS Pathogens, 22 pgs. PMID: 25811886. (link)

Welsby, Iain, et al. (2014). PARP12, an Interferon-stimulated Gene Involved in the Control of Protein Translation and Inflammation*. JBC, 16 pgs. PMID: 25086041. (link)

Duong, Sean, et al. (2014). Polymicrobial sepsis alters Ag-dependent and -independent memory CD8 T cell functions. Journal of Immunology. PMID: 24646738. (link)

Lu, Ben, et al. (2014). JAK/STAT1 signaling promotes HMGB1 hyperacetylation and nuclear translocation. PNAS, 6 pgs. PMID: 24469805. (link)

Munitic, Ivana, et al. (2013). Optineurin Insufficiency Impairs Interferon Regulatory Factor 3 (IRF3) but not NF-κB Activation in Immune Cells. Journal of Immunology. PMID: 24244017. (link)

Freeman, Bailey, et al. (2012). Regulation of innate CD8+ T-cell activation mediated by cytokines. PNAS, 6 pgs. PMID: 22665806. (link)

Sutherland, DB, et al. (2011). Evaluating vaccinia virus cytokine co-expression in TLR GKO mice. Immunology and Cell Biology. PMID: 21173782. (link)

Leu, Shaw-Wei, et al. (2011). TLR4 through IFN-β promotes low molecular mass hyaluronan-induced neutrophil apoptosis. Journal of Immunology, 8 pgs. PMID: 21098223. (link)

Yamada, Atashi, et al. (2009). IFN-γ down-regulates Secretoglobin 3A1 gene expression. Biochemical and Biophysical Research Communications. PMID: 19135978. (link)

Guo, Beichu, et al. (2008). The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice. JCI, 11 pgs. PMID: 18382764. (link)

Molle, Celine, et al. (2007). IL-27 Synthesis Induced by TLR Ligation Critically Depends on IFN Regulatory Factor 3. Journal of Immunology, 10 pgs. PMID: 17548596. (link)

Jaini, et al. (2006). Gene-Based Intramuscular Interferon-beta Therapy for Experimental Autoimmune Encephalomyelitis. Molecular Therapy, 7 pgs. PMID: 16782409. (link)

Braun, Benjamin, et al. (2006). Somatic activation of a conditional KrasG12D allele causes ineffective erythropoiesis in vivo. Blood Journal. PMID: 16720837. (link)

Zhang, F, et al. (2006). Differential growth of IFN-beta-engineered tumor cells in nude and IFN receptor-null mice. Journal of Interferon & Cytokine Research. PMID: 16487031. (link)

References: (For additional references, please refer to the Certificate of Analysis)

Mesplede et al. (2003) Autoimmunity 36(8):447.

Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461.

Higashi et al. (1983) J. Biol. Chem. 258(15):9522.

Hayashi et al. (2002) J. Immunol. 277(31):27880.

Fujimura et al. (2006) Infect. Immu. 75(5):2544.

Documentation

Comparison of Mouse IFN Beta with carrier and Mouse IFN Beta carrier-free

The activity of Mouse IFN Beta, carrier-free and Mouse IFN Beta with carrier was compared in the L929/EMCV CPE assay. The EC 50 for Mouse IFN Beta, carrier-free was 1.5 U/ml when calibrated to the international standard.

PBL/Mouse IFN Beta, carrier-free/1 x 105 units/12401-1

Mouse IFN Beta, carrier-free

Ordering

Specifications

Tech Info / Data

Citations

Documentation

Comparison of Mouse IFN Beta with carrier and Mouse IFN Beta carrier-free

PBL/Mouse IFN Beta, carrier-free/1 x 10⁵ units/12401-1