Features:- Peroxidase enzyme detection system- Elite® Avidin/biotin complex (ABC) technology- High sensitivity/low background- Approximately 5 times more sensitive - Used as a second label in multiple antigen labeling applications- CE Mark- No antibody included- ConcentrateKit Contents:- 2 ml Reagent A (Elite® Avidin DH) plus 2 ml Reagent B (Biotinylated HRP)Applications: - Immunohistochemistry- Immunocytochemistry - In situ hybridization- Blotting Applications- Elispot- ELISADetails:The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Standard) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. Advanced avidin/biotin technology:The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target.The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.This Standard kit consists of the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol only, and is intended to be paired with biotinylated secondary antibodies, such as LS-D1 (goat anti-rabbit IgG), LS-D2 (horse anti-mouse IgG), or LS-D3 (horse anti-goat IgG).Alternatively, this kit is available pre-packaged with various combinations of species specific blocking serum and biotinylated secondary antibody (see VECTASTAIN® Elite® ABC-HRP Staining Kits LS-J1019 thought LS-J1025).Also available in Ready-To-Use (R.T.U.) formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Staining Kit reagents (LS-J1026, LS-J1027, and LS-J1028).Immunohistochemistry protocol:Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting