- SynonymLILRA5,CD85f,ILT11,LIR9,LILRB7
- SourceBiotinylated Human LILRA5, His,Avitag (LI5-H82E1) is expressed from human 293 cells (HEK293). It contains AA Gly 42 - Arg 268 (Accession # A6NI73-1).Predicted N-terminus: Gly 42Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 28.9 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LILRA5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA5, His,Avitag (Cat. No. LI5-H82E1) with a linear range of 1.25-20 μg/mL (QC tested).
- BackgroundLIRs are immunoglobulin-like receptors that have activating and inhibitory functions in leukocytes. Leukocyte immunoglobulin-like receptor A5 (LILRA5) belongs to a family of receptors known to regulate leukocyte activation. LILRA5, expressed as a membrane-bound receptor and as a secreted molecule. The transmembrane LILRA5 contain a short cytoplasmic domain and a charged arginine residue within the transmembrane region that is likely to mediate its association with another coreceptor. LILRA5 is mostly expressed in myeloid cells, including monocytes and neutrophils.
- References
- (1)Shiroishi M., et al., 2006, J. Biol. Chem. 281:19536-19544.
- (2)Mitchell A., et al., 2008, Eur. J. Immunol. 38:3459-3473.
- (3)Borges L., et al., 2003, Blood. 101:1484-1486.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
