- SynonymMAD2L1,MAD2,HsMAD2
- SourceHuman MAD2L1, His Tag (MA1-H5123) is expressed from E.coli cells. It contains AA Met 1 -Asp 205 (Accession # NP_002349).Predicted N-terminus: Met 1Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 24.3 kDa. The protein migrates as 24 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human MAD2L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundMitotic arrest deficient 2-like protein 1 (MAD2L1) is also known as Mitotic spindle assembly checkpoint protein MAD2A (HsMAD2), MAD2. MAD2L1 / MAD2 belongs to the MAD2 family. MAD2L1 / MAD2 contains one HORMA domain. MAD2L1 is the component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 / MAD2 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
- References
- (1)Luo X., et al., 2000, Nat. Struct. Biol. 7:224-229.
- (2)Luo X., et al., 2002, Mol. Cell 9:59-71.
- (3)Luo X., et al., 2004, Nat. Struct. Mol. Biol. 11:338-345.
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