- SynonymTDGF1,CRGF,CRIPTO
- SourceHuman TDGF1, Fc Tag (CRO-H5253) is expressed from human 293 cells (HEK293). It contains AA Leu 31 - Thr 172 (Accession # P13385-1).Predicted N-terminus: Leu 31Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.6 kDa. The protein migrates as 44-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human TDGF1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundTeratocarcinoma-derived growth factor 1 (TDGF1) is also known as Cripto-1 growth factor (CRGF), Epidermal growth factor-like cripto protein CR1, CRIPTO, is a cell membrane which contains one EGF-like domain. TDGF1 is preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. TDGF1 interacts with the activin type-1 receptor ACVR1B. TDGF1 could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
- References
- (1)Bianco C., et al., 2002, Mol. Cell. Biol. 22:2586-2597.
- (2)Foley S.F., et al., 2003, Eur. J. Biochem. 270:3610-3618.
- (3)Sun C., et al., 2008, Biochem. Biophys. Res. Commun. 377:215-220.
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