주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
[주의] 동결된 세포주의 해동(Thawing)시, 세포 생존율 향상을 위하여collagen-coated plate 또는 flaks를 사용하십시오.추후 세포 배양 시에도 세포주의 부착율(adherence)이 좋지 않다면 collagen-coated plate 또는 flask를 사용하는것을 권장합니다.
Tet-Off and Tet-Off Advanced inducible cell lines are host cell lines that greatly simplify the task of setting up tetracycline-inducible geneexpression systems which are activated by removing doxycycline (Dox) from the culture medium (Figure 1; 1, 2). Our Tet-On®Advanced and Tet-On inducible cell lines are also available, which are activated by adding Dox to the culture medium.
□ These rigorously tested clonal cell lines:
● Stably express either the second-generation Tet-Off Advanced transactivator protein or the original Tet-Off
transactivator protein
● Allow you to induce high-level expression of your gene-of-interest by removing (Dox) from the culture of
transfected cells (Figure 2)
● Can save you up to two months of time in developing a complete Tet-controlled system.
Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTight in pTRE-Tight), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.Our PC12 Tet-Off Cell Line responds normally to nerve growth factor (NGF). For more details, see our report Neural Differentiation in Tet-Inducible PC12 Cell Lines.
□ References
1. Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89:5547-5551.2. Gossen, M., et al. (1995) Science 268:1766-1769.
□ Components
● Tet-Off or Tet-Off Advanced Cell Line
● CHO AA8-Luc Tet-Off Control Cell Line
● Tet System Approved FBS (50 ml)
