Kerafast/抗肌动蛋白-7抗体/EGA245/100ug

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¥6120.00
货号:EGA245
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品牌:Kerafast
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商品描述

ThesemousemonoclonalantibodiesweregeneratedagainstArABIdopsisthalianaActin-7andrecognizeA.thalianaActin-7.

Highlights:

  • ReactwithA.thalianaActin-7(Maydisplayreactivitytowardmultipleotherespecies)
  • SuitableforWesternBlot,ELISAandImmunofluorescenceapplications

Actin-7hasbeenidentifiedinanimals,plantsandprotists.Duringplantgrowthanddevelopment,thephytohormoneauxininducesawidearrayofchangesthatincludecelldivision,cellexpansion,celldifferentiation,andorganinitiation.IthasbeensuggestedthattheactinCytoskeletonplaysanactiveroleintheelaborationoftheseresponsesbydirectingspecificchangesincellmorphologyandcytoarchitecture.ThepromoterandproteinproductofACT7,anAraBDopsisvegetativeactingene,arerapidlyandstronglyinducedinresponsetoexogenousauxin.ACT7isrequiredfortheformationofcallustissue,andisalsoessentialforgerminationandrootgrowthACT7actingeneisalsoexpressedinrapidlydevelopingtissuesandrespondstoexternalstimuli,suchasexposuretohormones.

FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.

Specifications
ProductType: Antibody
Name: Actin-7Antibodies
AccessionID: P53492
Antigen: Actin-7
Isotype: IgG
Clonality: Monoclonal
CloneName: 29G12.G5.G6,33E8.C11.F5.D1,36H8.C12.H10.B6
Reactivity: TestedinA.thaliana.Maydisplayreactivitytowardmultipleotherespecies(ND)
Immunogen: A.thalianaActin-7
SpeciesImmunized: BALB/cmouse
PurificationMethod: ProteinG
Buffer: 0.1MSodiumPhosphate,pH7.4,0.15MNaCl,0.05%(w/v)SodiumAzide
TestedApplications: WB,ELISA,IF(ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.)
Storage: -20C
Shipped: Coldpacks
Provider
FromthelaboratoryofRichardB.Meagher,PhD,UniversityofGeorgia.
Comments
ItisadvisabletouseallthreeMAbsinthefirst-time,qualitativeexperimentalsetuptodeterminewhichisthemostsuitableforaspecificexperiment.ThesemaybeusedyocomplementexcperimentscarriedoutinvolvingmAbGEauniversalanti-actinsantibody.
References
  1. Kandasamy,M.K.,Gilliland,L.U.,McKinney,E.C.,andMeagher,R.B.(2001).Oneplantactinisovariant,ACT7,isinducedbyauxinandrequiredfornormalcallusformation.PlantCell13,1541-54.
  2. Kandasamy,M.K.,McKinney,E.C.,andMeagher,R.B.(1999).Thelatepollen-specificactinsinangiosperms.PlantJ18,681-91.
  3. Kandasamy,M.K.,McKinney,E.C.,Roy,E.,andMeagher,R.B.(2012).Plantvegetativeandanimalcytoplasmicactinssharefunctionalcompetenceforspatialdevelopmentwithprotists.PlantCell24,2041-57.
  4. L.U.Gilliland,L.C.Pawloski,M.K.Kandasamy,andR.B.Meagher,“ArabidopsisactingeneACT7playsanessentialroleingerminationandrootgrowth,”PlantJournal,vol.33,no.2,pp.319–328,2003.
  5. McDowell,JM,An,Y-q,Huang,S,McKinney,EC,MeagherRB:TheArabidopsisACT7ActinGeneIsExpressedinRapidlyDevelopingTissuesandRespondstoSeveralExternalStimuli.PlantPhysiol.(1996)111:699-711.

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Kerafast is a Boston-based reagent company whose primary mission is to make unique laboratory-made research tools easily accessible to the global scientific community. Our portfolio includes cell lines, antibodies, small molecules, dyes and more, many of which are not available elsewhere. Since our founding in 2011, researchers from more than 150 institutions worldwide have made their innovative reagents available through our online platform, where the materials can be quickly accessed without needing to go through the traditional Material Transfer Agreement process. We handle all selling and shipping logistics for the providing laboratory, as well as return generous royalty payments from every sale. We therefore help providing labs save time and resources, while generating extra funding for further research. Procuring scientists can more easily discover and access unique reagents that are often unavailable elsewhere, while also funding the work of other researchers.