Concentration20u/μl5 " . . . A C T↓A G T. . . 3 "3 " . . . T↑G AT C A . . . 5 "

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 1mM DTT,200μg/ml BSA and 50% glycerol.

Unit Definition20U(1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal InactivationNone

Quality Control AssaysLigation/ Recutting AssayAfter 20-fold overdigestion with Ahl I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 40U of Ahl I for 16 hours at 37˚C.

Ordering Information

Catalog No	Description	Pack Size
FDRE1118	Ahl I (Spe I)	25 preps

 

DownloadsManualAhl I (Spe I)

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。