Concentration20-50u/μl5"…R↓ AATTY…3"3"…YTTAA↑R…5"

Reaction Conditions 1X Buffer V4 10mM Tris-HCl (pH8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 50°C.

Storage Buffer10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal InactivationNone.

Ligation / Recutting Assay After 20-fold overdigestion with AcsI, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AcsI for 16 hours at 50°C.Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

Catalog No	Description	Pack Size
RE1114	AcsI {ApoI}	500u

 

DownloadsManualAcsI {ApoI}

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。