GradeMolecular Biology Grade

DescriptionDNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA and produce 3’-OH oligonucleotides in the presence of divalent cations. In the reaction solution, with the presence of Mg²⁺ helps the enzyme to produce nicks in double-stranded DNA, while with the presence of Mn²⁺, DNase I cleaves both strands of the DNA. DNase I is also useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. DNase I is offered as lyophilized powder with an activity of 500 Kunitz U/mg and is a chromatographically pure preparation. For greatest stability, DNase I is suggested to be dissolved at a concentration of at least 1mg/ml in 50% glycerol with 20mM Tris-HCl, pH7.5, and 1mM MgCl₂. This solution can be stored at -20°C for at least a year.

Specification

Appearance	White to off-white crystalline powder; a partially purified, lyophilized powder
Source	Bovine pancreas All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation
Unit Definition	One unit of DNase I is defined as the amount required to completely degrade 1µg of lambda DNA in 10 minutes at 37°C in 50µl of a buffer containing 40mM Tris-HCl (pH8.0), 2.5mM (up to 10mM) MgSO4, 1mM (up to 10mM) CaCl2. Under these assay conditions one unit of DNase activity is approximately equal to one Kunitz unit.

StorageStore at -20°C

 

Assay	Resuls
Activity	>500Kunitz U/mg
Loss on Drying	≤5.0%
Residue on Ignition	≤1.0%

Ordering Information

Catalog No	Description	Pack Size
PC0704-25mg	Deoxyribonuclease I (DNase I)	25mg
PC0704-50mg	Deoxyribonuclease I (DNase I)	50mg
PC0704-100mg	Deoxyribonuclease I (DNase I)	100mg
PC0704-1g	Deoxyribonuclease I (DNase I)	1g

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Deoxyribonuclease I (DNase I)

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