DescriptionViPrimePLUS One Step AtTaq RT-qPCR Master Mix is next generation first choice mix designed for fast and easy one step real-time PCR reaction set up. The improved formulation of master mix contains unique thermostable M-MULV enzyme, Hot Start Taq DNA Polymerases as well as MgCl2 and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature of 55°C, and has a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step AtTaq RT-qPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.ViPrimePLUS One Step AtTaq RT-qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved formulation of RT-qPCR master mix can detect extremely low copy number targets very specifically with high efficiency that gives CT values close to the theoretical time of detection. The ViPrimePLUS One Step AtTaq RT-qPCR Master Mix is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The formation of primer dimers and non-specific products is prevented leading to optimum sensitivity and specificity..ViPrimePLUS One Step AtTaq RT-qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS One Step AtTaq RT-qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.

ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

• One step real time RT-qPCR reaction set up
• Equipped with thermostable M-MULV enzyme and Hot Start Taq DNA Polymerases
• Reliable quantification of extremely low copy number targets
• Optimal performance for highly sensitive and specific RT-qPCR reaction
• Compatible with most of the real-time PCR platforms

Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”

StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step AtTaq RT-qPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Product Description	Compatible Hardware
QLMM03ViPrimePLUS One Step AtTaq RT-qPCR Master Mix	Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™
QLMM03-LRViPrimePLUS One Step AtTaq RT-qPCR Master Mix with Low ROX	Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7
QLMM03-RViPrimePLUS One Step AtTaq RT-qPCR Master Mix with ROX	Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS

Ordering Information

Catalog No	Description	Pack Size
QLMM03	ViPrimePLUS One Step AtTaq RT-qPCR MasterMix	150 reactions
QLMM03-LR	ViPrimePLUS One Step AtTaq RT-qPCR MasterMix with Low ROX	150 reactions
QLMM03-R	ViPrimePLUS One Step AtTaq RT-qPCR MasterMix with ROX	150 reactions

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ViPrimePLUS One Step AtTaq RT-qPCR MasterMix ViPrimePLUS One Step AtTaq RT-qPCR MasterMix with Low ROX ViPrimePLUS One Step AtTaq RT-qPCR MasterMix with ROX

PublicationThis Product Has Been Used In:Attaran, B., Falsafi, T. (2017). Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics, . World Journal of Gastroenterology, Vol. 23, No. 7. 1163-1170 (2017).

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。