DescriptionViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is next generation master mix designed for one step real-time PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases, EvaGreen® dye, as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. EvaGreen® dye in master mix is environmentally safe and highly stable which can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RT-qPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II in standard cycling conditions gives the industry leading performance in fast cycling conditions.

ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

• One step real time RT-qPCR reaction set up
• Equipped with thermostable M-MULV enzyme EvaGreen® dye – highest dye stability and safety
• Good buffer system for excellent amplification efficiency
• Minimal PCR inhibition
• Reliable quantification of low copy number targets
• Minimal PCR inhibition
• Optimal performance for highly sensitive and specific RT-qPCR reaction
• Compatible with most of the real-time PCR platforms

Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”

StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS Taq qPCR Green Master Mix II has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Product Description	Compatible Hardware
QLMM18ViPrimePLUS One Step Taq RT-qPCR Green Master Mix II (EvaGreen® Dye)	Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™
QLMM18-LRViPrimePLUS One Step Taq RT-qPCR Green Master Mix II with Low ROX (EvaGreen® Dye)	Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7
QLMM18-RViPrimePLUS One Step Taq RT-qPCR Green Master Mix II with ROX (EvaGreen® Dye)	Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS

Ordering Information

Catalog No	Description	Pack Size
QLMM18	ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II (EvaGreen® Dye)	150 reactions
QLMM18-LR	ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with Low ROX (EvaGreen® Dye)	150 reactions
QLMM18-R	ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with ROX (EvaGreen® Dye)	150 reactions

DownloadManual

ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II (EvaGreen® Dye) ViPrimePLUS One Step Taq RT-qPCR Green MasterMix II with Low ROX (EvaGreen® Dye) ViPrimePLUS Taq qPCR Green MasterMix II with ROX (EvaGreen® Dye)

PublicationThis Product Has Been Used In: Jahantigh, M., Mazaheri, M., Moghadam, A.Y., Motalleb, G., Najafi, S., Najafi, S., Pourrahmat, E., Rashki, A., Sabagh, K., Sanadgol, N., Talee, R. (2014).Epidermal growth factor receptor gene expression evaluation in colorectal cancer patients, Indian Journal of Cancer, Vol. 51, No. 3, 358-362 (2014).

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。