Vivantechnologys/ViPrimePLUS一步法Taq RT qPCR MasterMix/QLM13-LR

价格
面议
货号:QLMM13-LR
浏览量:127
品牌:VivanTechnologies
服务
全国联保
正品保证
正规发票
签订合同
商品描述

DescriptionViPrimePLUS One Step Taq RT-qPCR Master Mix is next generation master mix designed for one step realtime PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step Taq RT-qPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.ViPrimePLUS One Step Taq RT-qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RTqPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Master Mix is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.

ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

  • One step real time RT-qPCR reaction set up
  • Equipped with thermostable M-MULV enzyme
  • Good buffer system for excellent amplification efficiency
  • High sensitivity detection
  • Optimal performance for highly sensitive and specific RT-qPCR reaction
  • Compatible with most of the real-time PCR platforms

Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”

StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step Taq RTqPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Product DescriptionCompatible Hardware
QLMM13ViPrimePLUS One Step Taq RT-qPCR Master MixBiometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™
QLMM13-LRViPrimePLUS One Step Taq RT-qPCR Master Mix with Low ROXApplied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7
QLMM13-RViPrimePLUS One Step Taq RT-qPCR Master Mix with ROXApplied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS

Ordering Information

Catalog NoDescriptionPack Size
QLMM13ViPrimePLUS One Step Taq RT-qPCR MasterMix150 reactions
QLMM13-LRViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX150 reactions
QLMM13-RViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX150 reactions

DownloadManual

ViPrimePLUS One Step Taq RT-qPCR MasterMix ViPrimePLUS One Step Taq RT-qPCR MasterMix with Low ROX ViPrimePLUS One Step Taq RT-qPCR MasterMix with ROX

PublicationThis Product Has Been Used In:Attaran, B., Falsafi, T. (2017).). Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics, . World Journal of Gastroenterology, Vol. 23, No. 7. 1163-1170 (2017).

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物,预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶,反应缓冲液,dNTP,MgCl 2,惰性红色染料和稳定剂,这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1%琼脂糖上以大约400bp的速度迁移。