DescriptionViPrimePLUS AtTaq qPCR Master Mix is next generation first choice mix designed for fast and easy real-time PCR reaction set up. The improved formulation of master mix contains pure Hot Start Taq DNA Polymerases, highest quality dNTPs and buffer components at optimal concentrations. Hot Start Taq DNA Polymerases in the master mix provide antibody mediated hot start mechanism which releases more active enzyme and requires shorter activation time to achieve excellent results in reaction efficieny, correlation coefficient and slope.ViPrimePLUS AtTaq qPCR Master Mix can be used to amplify any DNA template including genomic, cDNA and viral sequences. The improved formulation of qPCR master mix can detect extremely low copy number targets very specifically with high efficiency. The qPCR master mix is designed to prevent and reduce the formation of primer dimers and non-specific products leading to optimum sensitivity and specificity.ViPrimePLUS AtTaq qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS AtTaq qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.

ApplicationsAll kinds of sample material suited for qPCR amplification can be used.

Features

• Fast and easy real-time PCR reaction set up
• Rapid extension rate for early Ct values
• Contain Hot Start Taq DNA Polymerase - highest sensitivity and specificity
• Increased limit of detection
• Compatible with most of the real-time PCR platforms

Component1.6ml aliquots of master mix

StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS AtTaq qPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Product Description	Compatible Hardware
QLMM01ViPrimePLUS AtTaq qPCR Master Mix	Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™
QLMM01-LRViPrimePLUS AtTaq qPCR Master Mix with Low ROX	Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7
QLMM01-RViPrimePLUS AtTaq qPCR Master Mix with ROX	Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS

Ordering Information

Catalog No	Description	Pack Size
QLMM01	ViPrimePLUS AtTaq qPCR MasterMix	150 reactions
QLMM01-LR	ViPrimePLUS AtTaq qPCR MasterMix with Low ROX	150 reactions
QLMM01-R	ViPrimePLUS AtTaq qPCR MasterMix with ROX	150 reactions

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ViPrimePLUS AtTaq qPCR Master Mix ViPrimePLUS AtTaq qPCR Master Mix with Low Rox ViPrimePLUS AtTaq qPCR Master Mix with Rox

VivanTechnologies的2X ViRed Taq Master Mix是一种优化的即用型2X浓缩DNA扩增混合物，预先混合了单色染料–红色示踪染料。2X ViRed Taq预混液包含 Taq DNA聚合酶，反应缓冲液，dNTP，MgCl 2，惰性红色染料和稳定剂，这些都是常规DNA扩增以获得最多8kb的PCR和DNA产物所需的。惰性红色染料和稳定剂可将最终产物直接加载到电泳凝胶上。这种红色染料不会影响扩增以及其他下游应用。红色染料在1X TBE缓冲液中的1％琼脂糖上以大约400bp的速度迁移。