c-Myc is a transcription factor that belongs to the Myc family of proteins consisting of c-, N-, and L-Myc. Myc proteins are members of the basic helix-loop-helix (bHLH) protein family, in which the HLH domain is responsible for dimerization and the adjacent basic region mediates sequence-specific DNA binding. Myc has generally been associated with the promotion of cellular growth and proliferation, desensitization to growth-inhibitory stimuli, blockade of cell differentiation, cellular immortalization, and oncogenic transformation, as well as sensitization to apoptosis-inducing signals. Myc has also been linked to the regulation of various metabolic pathways, the induction of DNA damage, and genomic instability. In normal cells, c-Myc expression is generally induced by mitogens and suppressed by growth-inhibitory signals. Oncogenic activation of c-Myc occurs by direct gene alterations, such as translocation or amplification, or by mutations in upstream signaling pathways. These accidents commonly result in deregulated and/or elevated expression of c-Myc and its product. The activated c-Myc protein regulates multiple targeted genes through binding at the consensus DNA sequence CACGTG or E box.

Upon receipt, the positive control should be removed and stored at -20^° or -80^°C. The remainder of the kit can be stored for up to 6 months at 2-8^°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2^° to 8^°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20^°C upon receipt. Avoid repeated freeze-thaw cycles.