品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
- IGA
- IGG2A
- IGG2B
- IGHG1
- IGM
Assay Format
Number of Targets Detected:
8
Species Detected:
- Rat
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
16, 32, or 64 Sample Kit
Product Feature
- One step rat monoclonal antibody isotyping
- Use only 1-2 µl sample
- The whole experiment can be done within 1 hour
- Sandwich based technology for high specificity and sensitivity
- Low system CV with high reproducibility
- High throughput sample processing
- Qualitative visual inspection or semi-quantitative result
- Processed slides can be stored for years without signal decay
Target Name
Heavy Chains: IgA, IgM, IgG1, IgG2a, IgG2b, IgG2cLight Chains: Kappa, Lambda
Application Notes
Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components
- Rat Ig Rapid Isotyping Array Glass Slide(s)
- Sample Diluent
- 20X Wash Buffer
- Labeled Detection Antibody
- Slide Washer/Dryer
- 96-well Plate
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Mix samples with detection antibodies (in 96-well plate)
- Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
Upon receipt, all components of the Isotyping Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.
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Manual
SDS
GAL
Analysis Tool
