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0512-67156496
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商品描述
Antigen Information
Gene Symbols:
- ATF2
- FOS
- JUN
- MAP3K7
- SMAD1
- SMAD2
- SMAD4
- SMAD5
Assay Format
Pathway:
- PKC Signaling
- TGF-beta Signaling
Number of Targets Detected:
8
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
c-Jun (P-Ser73)
| SMAD1 (P-Ser463/465)
| SMAD5 (P-Ser463/465)
| SMAD4 (P-Thr277)
| ATF2 (P-Thr69/71)
|
C-Fos (P-Thr232)
| SMAD2 (P-Ser245/250/255)
| TAK1 (P-Ser412)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human TGF beta Phosphorylation Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 500X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE"s ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Typical Data
Figure 1 - HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Calyculin A for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system. 
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Poling, Holly M., et al. "Mechanically induced development and maturation of human intestinal organoids in vivo." Nature biomedical engineering 2.6 (2018): 429-442.Species: HumanSample type: Tissue Lysates
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Manual
SDS
Analysis Tool
