品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
CCL20 | CSF2 | IFNG | IFNL2 | IL10 | IL12A | IL13 | IL17A | IL17F | IL1B| IL2 | IL21 | IL22 | IL23A | IL4 | IL5 | IL6 | LTA | TGFB1 | TNF View more Hide
Assay Format
Number of Targets Detected:
20
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 beta(IL-1 F2)
| IL-10
| IL-12 p70
|
IL-13
| IL-17A
| IL-17F
| IL-2
| IL-21
|
IL-22
| IL-23
| IL-28A(IFN-lambda 2)
| IL-4
| IL-5
|
IL-6
| MIP-3 alpha(CCL20)
| TGF beta 1
| TNF alpha
| TNF beta(TNFSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Th1/Th2/Th17 Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Du X., Shi R., Wang Y., et al. Isoforskolin and forskolin attenuate lipopolysaccharide-induced inflammation through TLR4/MyD88/NF-?B cascades in human mononuclear leukocytes. Phytother Res. 2019 Jan 14. doi: 10.1002/ptr.6248.Species: HumanSample type: Conditioned Media
- Canete J., Celis R., Yeremenko N., et al. Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis. Arthritis Res Ther. 2015 Jul 9;17:173. doi: 10.1186/s13075-015-0688-0.Species: HumanSample type: Synovial Fluid
- Gomez-Puerta J., et al. Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and -negative rheumatoid arthritis patients. GÃ?mez-Puerta et al. Arthritis Research & Therapy 2013, 15:R182Species: HumanSample type: Synovial Fluid
- Kotlarz D, Zi?tara N, Uzel G, et al. Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome. J. Exp. Med. 2013 Mar 11;210(3):433-443.Species: HumanSample type: Conditioned Media
- Yang P., Foster CS. Interleukin 21, interleukin 23, and transforming growth factor ?1 in HLA-A29-associated birdshot retinochoroidopathy. Am J Ophthalmol. 2013 Aug;156(2):400-406.e2. doi: 10.1016/j.ajo.2013.03.004.Species: HumanSample type: Serum
- Gómez-Puerta, José A., et al. "Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and?negative rheumatoid arthritis patients."?Arthritis research & therapy?15.6 (2013): R182.Species: HumanSample type: Synovial fluid
- Liu R, Wu Q, Su D, et al. A regulatory effect of IL-21 on T follicular helper-like cell and B cell in rheumatoid arthritis. Arthritis Research & Therapy. 2012;14(6):R255. doi:10.1186/ar4100.Species: HumanSample type: Serum
- Celis R., et al. Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features. Celis et al. Arthritis Research & Therapy 2012, 14:R93Species: HumanSample type: Synovial Fluid
- Gomez-Puerta J., et al. Differences in synovial fluid cytokine levels but not in synovial tissue cell infiltrate between anti-citrullinated peptide/protein antibody-positive and -negative rheumatoid arthritis patients. Gómez-Puerta et al. Arthritis Research & Therapy 2013, 15:R182Species: HumanSample type: Synovial Fluid
No posts found
Manual
SDS
GAL
Analysis Tool
