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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Antigen Information
Gene Symbols:
- MMP1
- MMP10
- MMP13
- MMP2
- MMP3
- MMP8
- MMP9
- TIMP1
- TIMP2
- TIMP4
Assay Format
Pathway:
- Akt Signaling
- HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Other Body Fluids
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
MMP-1
| MMP-10
| MMP-13
| MMP-2
| MMP-3
|
MMP-8
| MMP-9
| TIMP-1
| TIMP-2
| TIMP-4
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human MMP Array Q1 Slide(s)
- Sample Diluent
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
- Sava P., Ramanathan A., Dobronyi A., et al. Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung. JCI Insight. 2017 Dec 21;2(24). pii: 96352. doi: 10.1172/jci.insight.96352Species: HumanSample type: Conditioned Media
- Cekanova M., Feranando RI., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016. Species: HumanSample type: Cell Lysate
- Collazos J., Asensi V., Martin G., et al. The effect of gender and genetic polymorphisms on matrix metalloprotease (MMP) and tissue inhibitor (TIMP) plasma levels in different infectious and non-infectious conditions. Clin Exp Immunol. 2015 Nov;182(2):213-9. doi: 10.1111/cei.12686.Species: HumanSample type: Plasma
- Tian F., Qin C., Li X., et al. Decreased stathmin-1 expression inhibits trophoblast proliferation and invasion and is associated with recurrent miscarriage. Am J Pathol. 2015 Oct;185(10):2709-21. doi: 10.1016/j.ajpath.2015.06.010. Species: HumanSample type: Conditioned Media
- Cekanova M., Fernando R., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016. Species: HumanSample type: Conditioned Media
- Chia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6Species: HumanSample type: Conditioned Media
- Epanchintsev A., Pavithra S., Verma R., Lyakhovich A. IL-6, IL-8, MMP-2, MMP-9 are overexpressed in Fanconi anemia cells through a NF-?B/TNF-? dependent mechanismSpecies: HumanSample type: Conditioned Media
- Martin G., Asensi V., Montes H., Collazos J., Alvarez V., Carton J., Taboada F., Valle-Garay E. Role of plasma matrix-metalloproteases (MMPs) and their polymorphisms (SNPs) in sepsis development and outcome in ICU patients. Scientific Reports 4, 5002. doi:10.1038/srep05002.Species: HumanSample type: Plasma
- Wang Y., et al. Circulating matrix metalloproteinase patterns in association with aortic dilatation in bicuspid aortic valve patients with isolated severe aortic stenosis. Heart and Vessels, October 2014, epub ahead of print. DOI 10.1007/s00380-014-0593-5Species: HumanSample type: Plasma
- Yang C-C, Lin C-Y, Wang H-S, Lyu S-R (2013) Matrix Metalloproteases and Tissue Inhibitors of Metalloproteinases in Medial Plica and PannuslikeTissue Contribute to Knee Osteoarthritis Progression. PLoS ONE 8(11): e79662. doi:10.1371/journal.pone.0079662Species: HumanSample type: Tissue Lysate
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