9-tert-Butyl Doxycycline (9TB) is a Tet On/Off system agonist capable of activating and acting as an inducer for the tetracycline-transactivator (tTA) and reverse tTA (rtTA), and tTA-responsive promoters (Ptets), herein known as the Tet switch. The compound is able to activate the Tet switch with approximately 10-fold greater efficacy, especially in lipophilic environments such as the brain and lung and other similar biocompartments. 9TB is freely soluble in water, and can be administered IV, IP and in the drinking water of experimental animals, allowing for ready dosing. 9TB also has found use in studying hypoxia-regulated gene expression on cell survival using the tetracycline-inducible (tet-on) system, where exposure to the inducing ligand doxycycline (dox) inhibited caspase-3 cleavage in mitochondria in

Publications

1) Zhu, P. et al., “Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons”, PLoS ONE. 2007; 2(6): e533.2) Halterman, M.W. “An Improved Method for the Study of Apoptosis-Related Genes Using the Tet-On System” J. Biomol. Screen. 2011; 16(3): 332-3373) Jiang, D. et al. “Airway Epithelial NF-kB Activation Promotes Mycoplasma pneumoniae Clearance in Mice” PLoS ONE. 2012; 7(12): e52969.4)Furutachi, S. et al. “Slowly dividing neural progenitors are an embryonic origin of adult neural stem cells”, Nature Medicine, 2015; doi:10.1038/nn.3989.5) Furlan, G., et al. (2017). “Life-Long Neurogenic Activity of Individual Neural Stem Cells and Continuous Growth Establish an Outside-In Architecture in the Teleost Pallium.” Current Biology 27(21): 3288-3301. DOI:10.1016/j.cub.2017.09.0526) Alonso, S. B., et al. (2019). “An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants.” The EMBO Journal 38: e98791. DOI:10.15252/embj.201798791

Categories	Biochemical Reagents
Filter	Tetracycline
CAS Number	233585-94-9
Molecular Formula	C26H33ClN2O8
Molecular Weight (g/mol)	536.19 HCl salt
Purity	>90%
Storage	-20 °C or below

Echelon常用产品报价单产品名货号公司分类商城分类美金价Echelon/Anti-Rat F1-ATP Synthase Alpha Subunit Rabbit Polyclonal Antibody/100 UL/Z-RATPAZ-RATPA功能性抗体400.00Echelon/Biotinylated Anti-PtdIns(3,4)P2 IgG/50 UG/Z-B034Z-B034其它重组蛋白586.00Echelon/Goat anti-human Autotaxin antibody/10 UG/Z-P400Z-P400抗人83.00Echelon/PI4-Kinase beta (PI4Kb), active/5 UG/P32-102GP32-102G激酶226.00Echelon/1-Methylhistamine dihydrochloride/25 MG/B-0032B-0032其他试剂154.00Echelon/Neutral Sphingomyelinase Assay Service/10 SAMPLES/T-1800T-1800脂类663.00Echelon/PI3-K Beta Inhibitor Screening Service/60 Samples/T-1300B-4T-1300B-4脂质代谢研究3407.00Echelon/Cardiolipin Beads/100 UL/P-BCLPP-BCLP微球123.00Echelon/PI3-K Alpha Inhibitor Screening Service/40 SAMPLE/T-1300A-3T-1300A-3多肽2966.00Echelon/5′ PtdIns(3,4,5)P3 Phosphatase Activity Fluorescence Polarization Assay/EA KIT/K-1400K-1400其它检测试剂盒557.00echelon-inc/Sphingosine 1-Phosphate ELISA Kit/1ea/K-1900-1eaK-1900-1eaInhibitors夹心法ELISA1214.00