Echelon’s Malachite Green Assay Kit is designed for the in vitro measurement of lipid and protein phosphatase activity. The liberated phosphate forms a colored complex with molybdate/malachite green, which is quantified by reading the absorbance at 620 nm. The assay uses water-soluble lipid substrates so there is no need to make phospholipid vesicles. The assay is fast, convenient and offers a flexible non-radioactive alternative for measuring specific phosphatase activities. The Malachite Green Assay kit can measure as little as 50 pmoles of free phosphate and has a high level of accuracy in the range of 200 to 2,000 pmol of phosphate per assay point. The kit includes enough reagents for 192 samples, standards, and controls.

The kit is offered in three sizes:

1. K-1500: includes 2 X 96-well plates, 1 vial of phosphate standard and a bottle of malachite green solution.
2. K-1520: includes 1 X K-1500 + your choice of 2 X 100 ug lipid substrates.
3. K-1530: includes 1 X K-1500 + your choice of 3 X 100 ug lipid substrates.

Suggested available substrates: diC8 PI(3)P, PI(4)P, PI(5)P, PI(3,4)P2, PI(3,5)P2, PI(4,5)P2, PI(3,4,5)P3

The Malachite Green Solution can also be purchased separately.

Phosphatase Substrate	Lipid Phosphatase Enzyme
PtdIns(3)P	Myotubularin, MTMR2
PtdIns(4)P
PtdIns(5)P
PtdIns(3,4)P2	PTEN
PtdIns(3,5)P2	OCRL1, 5Ptase IV, Synaptojanin
PtdIns(4,5)P2	MTMR2, PTPRQ
PtdIns(3,4,5)P3	PTEN, SHIP1, SHIP2, SKIP, PTPRQ, 5ptase II

Categories	Kits & Assays
Filter	Activity, Phosphatase

Echelon常用产品报价单产品名货号公司分类商城分类美金价Echelon/Anti-Rat F1-ATP Synthase Alpha Subunit Rabbit Polyclonal Antibody/100 UL/Z-RATPAZ-RATPA功能性抗体400.00Echelon/Biotinylated Anti-PtdIns(3,4)P2 IgG/50 UG/Z-B034Z-B034其它重组蛋白586.00Echelon/Goat anti-human Autotaxin antibody/10 UG/Z-P400Z-P400抗人83.00Echelon/PI4-Kinase beta (PI4Kb), active/5 UG/P32-102GP32-102G激酶226.00Echelon/1-Methylhistamine dihydrochloride/25 MG/B-0032B-0032其他试剂154.00Echelon/Neutral Sphingomyelinase Assay Service/10 SAMPLES/T-1800T-1800脂类663.00Echelon/PI3-K Beta Inhibitor Screening Service/60 Samples/T-1300B-4T-1300B-4脂质代谢研究3407.00Echelon/Cardiolipin Beads/100 UL/P-BCLPP-BCLP微球123.00Echelon/PI3-K Alpha Inhibitor Screening Service/40 SAMPLE/T-1300A-3T-1300A-3多肽2966.00Echelon/5′ PtdIns(3,4,5)P3 Phosphatase Activity Fluorescence Polarization Assay/EA KIT/K-1400K-1400其它检测试剂盒557.00echelon-inc/Sphingosine 1-Phosphate ELISA Kit/1ea/K-1900-1eaK-1900-1eaInhibitors夹心法ELISA1214.00