TheEchelonautotaxinactivityassayserviceusestheAutotaxinActivityAssayKit(cat#K-4100).ThiskitusesthefluorogenicautotaxinsubstrateFS-3,anLPCanaloguethatisconjugatedwithbothafluorophore(fluorescein)andaquencher.Initsnativestatethequencherinterfereswithfluorescein’sfluorescence.OnceautotaxincleavesFS-3,fluoresceinbecomesliberatedfromthequencher,resultinginincreasedfluorescence.

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ThisassaygivesaquantitativemeasurementofautotaxinactivityinBIOLOGicalsamplesbymeasuringchangesinfluorescenceovertime.MeasurementsarereportedinFS-3units.Themassofautotaxininthesampleisnotgiven,becausethesameamountofautotaxinindifferentbiologicalsamplescanhavevastlydifferentenzymaticactivitiesduetothepresenceofotherfactors.Samplesarerunat0.5xinordertominimallydilutethesefactorsandmoreaccuratelyreflectenzymaticactivityinthebiologicalsituation.

SampleConsiderations: TheAutotaxinActivityAssayServicehasbeentestedforthequantificationofautotaxinactivityinserum,plasmaandcellculturemedia.Pleaserefertothetechnicaldatasheetformoreinformation.Toplaceanorderpleasecontactusat801-588-0455.

NoticetoPurchaser:EchelonBiosciencesproductsaresoldforresearchanddevelopmentpurposesonlyandarenottobeincorporatedintoproductsforresalewithoutwrittenpermissionfromEchelonBiosciences.ThecompoundFS-3anditsuseinassayingforLysophospholipaseDactivityarecoveredbyEchelonBiosciencesInc.USpatent7,989,663.Thepurchaseofthisproductincludesalimited,non-transferableimmunityfromsuitundertheforegoingpatentclaimsforusingonlythisamountofproductforthepurchaser’sowninternalresearch.Forinquiriesemailbusdev@echelon-inc.com

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Categories	Services
Filter	Activity,Autotaxin

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