iLite ADCC Target EGFR Positive Assay Ready Cells

iLite ADCC Target EGFR Positive Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite Target EGFR (+) Assay Ready Cells are intended for testing:

• ADCC activity
• anti-EGFR ADCC activity

Content: >250 μL of Assay Ready Cells suspended in cryoprotective medium from Gibco
Receipt and Storage: Upon receipt confirm that adequate dry-ice is present, and the cells are frozen. Immediately transfer to -80°C storage. Cells should be stored at -80°C (do not store at any other temperature) and are stable as supplied until the expiry date shown. Cells should be used within 30 min of thawing.
For Research Use Only

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Key benefits of iLite® Assays

• Highly specific reporter gene cell lines
• Very sensitive cell line responses (>10 fold inductions)
• Assay Ready Cells – ready-to-use from the freezer, without culturing of cells
• Assays within a workday (typically 4-7 hour assays)
• Normalization gene, which eliminates unwanted matrix effects

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Background

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism whereby pathogenic cells are lysed by lymphocytes, most often Natural Killer (NK) cells. The mechanism involves binding of antibodies to surface antigens on the pathogen. Crosslinking of these antibodies to NK cells through the binding of the Fc-portion to Fc receptors on the NK cells leads to activation of the NK cell and formation of an immune synapse with the pathogenic cell. The NK cell releases cytotoxic granules containing granzymes and perforin into the synapse, leading to apoptosis of the targeted cell (1).

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Related Products

iLite Effector V Assay Ready Cells
iLite ADCC Target EGFR (-)
iLite ADCC anti-EGFR Activity Set

EagleBio的iLite®分析就绪细胞iLite®  技术基于报告基因检测格式，经过修改和适用于整个药物开发周期中的应用以及生物药物的监测。这些细胞系可以开发用于任何生物制药靶标，并且可以使用相同的细胞系轻松设置药物效力（即药物活性）的测定方法。检验就绪的细胞经过基因工程处理，可与报告基因检验技术一起使用，以检测药物效力和NAb。